Overview

  • Product nameAnti-53BP1 antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1
  • SpecificityThis antibody is specific for 53BP1 protein.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide representing a portion of human 53BP1 encoded exon 19 (LocusLink ID 7158).

  • Positive control
    • U205, 293T and MO59K cell lysates (human). MEF lysates (mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab36828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/200 - 1/1000.

Target

  • FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 antibody images

  • Anti-53BP1 antibody (ab36828) at 1/2000 dilution + Whole cell lysate from human U205 cells at 20 µg
    Developed using the ECL technique

    Predicted band size : 214 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)

References for Anti-53BP1 antibody (ab36828)

This product has been referenced in:
  • Han W  et al. Nitric oxide mediated DNA double strand breaks induced in proliferating bystander cells after alpha-particle irradiation. Mutat Res 684:81-9 (2010). ICC/IF ; Chinese Hamster . Read more (PubMed: 20026341) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.


I have checked our catalog regarding your search for an p53bpi antibody which binds in the
tudor domain of this protein.

Unfortunately, we have antibodies which will bind the N-or the C-Terminu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"