Recombinant
RabMAb

Anti-53BP1 antibody [EPR2173(2)] (ab175188)

Overview

  • Product name
    Anti-53BP1 antibody [EPR2173(2)]
    See all 53BP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2173(2)] to 53BP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 53BP1 aa 900-1000 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q12888

  • Positive control
    • HepG2 lysates. Human fetal brain and heart lysates. Human cervical carcinoma and prostate hyperplasia tissue. HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 214 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
    • Involvement in disease
      Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
    • Sequence similarities
      Contains 2 BRCT domains.
    • Post-translational
      modifications
      Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
      Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • 53 BP1 antibody
      • 53BP1 antibody
      • FLJ41424 antibody
      • MGC138366 antibody
      • p202 antibody
      • p53 binding protein 1 antibody
      • p53 BP1 antibody
      • p53-binding protein 1 antibody
      • p53BP1 antibody
      • TP53 BP1 antibody
      • TP53B_HUMAN antibody
      • Tp53bp1 antibody
      • TRP53 BP1 antibody
      • Tumor protein 53 binding protein 1 antibody
      • Tumor protein p53 binding protein 1 antibody
      • Tumor suppressor p53 binding protein 1 antibody
      • Tumor suppressor p53-binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-53BP1 antibody [EPR2173(2)] (ab175188) at 1/50000 dilution

      Lane 1 : Human fetal heart lysates
      Lane 2 : HepG2 lysates
      Lane 3 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 214 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling 53BP1 with ab175188 at a 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling 53BP1 with ab175188 at a 1/50 dilution.

    • Immunofluorescence analysis of HepG2 cells labeling 53BP1 with ab175188 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Han J  et al. BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability. Nat Commun 8:1470 (2017). WB . Read more (PubMed: 29133916) »
    • Lou J  et al. AUNIP/C1orf135 directs DNA double-strand breaks towards the homologous recombination repair pathway. Nat Commun 8:985 (2017). Read more (PubMed: 29042561) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 22 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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