Anti-53BP1 antibody [EPR2173(2)] (ab175188)
- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-53BP1 antibody [EPR2173(2)]
See all 53BP1 primary antibodies -
DescriptionRabbit monoclonal [EPR2173(2)] to 53BP1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 53BP1 aa 900-1000 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12888 -
Positive control
- HepG2 lysates. Human fetal brain and heart lysates. Human cervical carcinoma and prostate hyperplasia tissue. HepG2 cells.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR2173(2)
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000 - 1/200000. Predicted molecular weight: 214 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
-
FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
-
Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
-
Sequence similaritiesContains 2 BRCT domains.
-
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
- Information by UniProt
-
Database links
- Entrez Gene: 7158 Human
- Omim: 605230 Human
- SwissProt: Q12888 Human
- Unigene: 440968 Human
-
Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
-
![Western blot - Anti-53BP1 [EPR2173(2)] antibody (ab175188)](http://a.abcam.com/ps/products/175/ab175188/Images/ab175188-201489-ab1751881.jpg)
Western blot - Anti-53BP1 [EPR2173(2)] antibody (ab175188)All lanes : Anti-53BP1 antibody [EPR2173(2)] (ab175188) at 1/50000 dilution
Lane 1 : Human fetal heart lysates
Lane 2 : HepG2 lysates
Lane 3 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 214 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 [EPR2173(2)] antibody (ab175188)](http://a.abcam.com/ps/products/175/ab175188/Images/ab175188-201488-ab1751882.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 [EPR2173(2)] antibody (ab175188)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling 53BP1 with ab175188 at a 1/50 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 [EPR2173(2)] antibody (ab175188)](http://a.abcam.com/ps/products/175/ab175188/Images/ab175188-201487-ab1751883.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 [EPR2173(2)] antibody (ab175188)Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling 53BP1 with ab175188 at a 1/50 dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-53BP1 [EPR2173(2)] antibody (ab175188)](http://a.abcam.com/ps/products/175/ab175188/Images/ab175188-201486-ab1751884.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-53BP1 [EPR2173(2)] antibody (ab175188)Immunofluorescence analysis of HepG2 cells labeling 53BP1 with ab175188 at a 1/100 dilution.
Protocols
References
This product has been referenced in:
- Han J et al. BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability. Nat Commun 8:1470 (2017). WB . Read more (PubMed: 29133916) »
- Lou J et al. AUNIP/C1orf135 directs DNA double-strand breaks towards the homologous recombination repair pathway. Nat Commun 8:985 (2017). Read more (PubMed: 29042561) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"