Anti-53BP1 (phospho S25) antibody (ab70323)


  • Product nameAnti-53BP1 (phospho S25) antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1 (phospho S25)
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A phosphorylated synthetic peptide representing a portion of human 53BP1 around serine 25 (NP_005648.1.)

  • Positive control
    • Whole cell lysate from 293T cells treated with NCS (200 ng/ml for 1h).



Our Abpromise guarantee covers the use of ab70323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa).
IP Use at 1-4 µg/mg of lysate.
Flow Cyt Use 0.25µg for 106 cells.

0.25 μg per 1 million cells in a 150 mcl reaction.

ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 (phospho S25) antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells, untreated
    Lane 2 : Whole cell lysate from 293T cells treated with NCS (200 ng/ml for 1h)

    Lysates/proteins at 50 µg per lane.

    Predicted band size : 214 kDa
    Observed band size : >220 kDa (why is the actual band size different from the predicted?)

References for Anti-53BP1 (phospho S25) antibody (ab70323)

ab70323 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton-X100 in PBS
Fixative Paraformaldehyde

Dr. Kirk McManus

Verified customer

Submitted Aug 20 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (whole embryos (~10 embryos per lane))
Loading amount 200000 cells
Specification whole embryos (~10 embryos per lane)
Treatment X-rays (0, 25 and 50 Gy)
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 20 2009