Anti-53BP1 (phospho T1609 + S1618) antibody (ab191551)

Overview

  • Product name
    Anti-53BP1 (phospho T1609 + S1618) antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1 (phospho T1609 + S1618)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human 53BP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab191551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 214 kDa.

Target

  • Function
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Images

  • All lanes : Anti-53BP1 (phospho T1609 + S1618) antibody (ab191551) at 1/1000 dilution

    Lane 1 : MEFs (negative control)
    Lane 2 : HeLa cell mitotic extract (positive control)
    Lane 3 : HeLa cell G1 extract (low phosphorylation)

    Predicted band size: 214 kDa

  • Lane 1 : Anti-53BP1 (phospho T1609 + S1618) antibody (ab191551) at 1/500 dilution
    Lane 2 : Anti-53BP1 (phospho T1609 + S1618) antibody (ab191551) at 1/1000 dilution
    Lane 3 : Anti-53BP1 (phospho T1609 + S1618) antibody (ab191551) at 1/2000 dilution

    All lanes : HeLa cell mitotic extracts

    Predicted band size: 214 kDa

References

ab191551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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