Overview

  • Product name
  • Description
    Rabbit polyclonal to 53BP2/ASPP2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 825 and 875 of human 53BP2/ASPP2 (NP_001026855.1)

  • Positive control
    • Human prostate adenocarcinoma and mouse squamous cell carcinoma tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
    • Tissue specificity
      Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
    • Sequence similarities
      Belongs to the ASPP family.
      Contains 2 ANK repeats.
      Contains 1 SH3 domain.
    • Domain
      The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
    • Cellular localization
      Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
    • Information by UniProt
    • Database links
    • Alternative names
      • 53BP2 antibody
      • Apoptosis stimulating of p53 protein 2 antibody
      • Apoptosis stimulating protein of p53 2 antibody
      • Apoptosis-stimulating of p53 protein 2 antibody
      • ASPP2 antibody
      • ASPP2_HUMAN antibody
      • BBP antibody
      • Bcl2 binding protein antibody
      • Bcl2-binding protein antibody
      • NY REN 51 antigen antibody
      • p53 binding protein 2 antibody
      • p53-binding protein 2 antibody
      • p53BP2 antibody
      • PPP1R13A antibody
      • Renal carcinoma antigen NY-REN-51 antibody
      • Tp53bp2 antibody
      • Tumor protein p53 binding protein 2 antibody
      • Tumor suppressor p53 binding protein 2 antibody
      • Tumor suppressor p53-binding protein 2 antibody
      see all

    Anti-53BP2/ASPP2 antibody images

    • ab84996 at 1/500 dilution staining 53BP2/ASPP2 in human prostate adenocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84996 at 1/100 dilution staining 53BP2/ASPP2 in mouse squamous cell carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References for Anti-53BP2/ASPP2 antibody (ab84996)

    ab84996 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84996.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up