Biological descriptionPotent NMDA receptor glycine site antagonist. Water soluble form available - see (ab120254).
Chemical name5,7-Dichloro-4-hydroxyquinoline-2-carboxylic acid
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in 1 eq. NaOH to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab12416 staining cGMP in SKNSH cells treated with 5,7-Dichlorokynurenic acid (ab120023), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 5,7-Dichlorokynurenic acid, as described in literature. The cells were incubated at 37°C for 20 minutes in media containing different concentrations of ab120023 (5,7-Dichlorokynurenic acid) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for 5,7-Dichlorokynurenic acid (ab120023)
This product has been referenced in:
Baron BM et al. [3H]5,7-dichlorokynurenic acid, a novel radioligand labels NMDA receptor-associated glycine binding sites. Eur J Pharmacol206:149-54 (1991).
Read more (PubMed: 1829684) »
Baron BM et al. Activity of 5,7-dichlorokynurenic acid, a potent antagonist at the N-methyl-D-aspartate receptor-associated glycine binding site. Mol Pharmacol38:554-61 (1990).
Read more (PubMed: 2172769) »