5,7-Dichlorokynurenic acid sodium salt (ab120254)

Overview

  • Product name
    5,7-Dichlorokynurenic acid sodium salt
  • Description
    NMDA receptor glycine site antagonist; water soluble
  • Biological description
    Potent NMDA receptor glycine site antagonist. Water soluble form.

    Also available in simple stock solutions (ab146691) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab12416 staining cGMP in SKNSH cells treated with 5,7-Dichlorokynurenic acid sodium salt (ab120254), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 5,7-Dichlorokynurenic acid sodium salt, as described in literature.
    The cells were incubated at 37°C for 20 minutes in media containing different concentrations of ab120254 (5,7-Dichlorokynurenic acid sodium salt) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Baron BM  et al. [3H]5,7-dichlorokynurenic acid, a novel radioligand labels NMDA receptor-associated glycine binding sites. Eur J Pharmacol 206:149-54 (1991). Read more (PubMed: 1829684) »
  • Baron BM  et al. Activity of 5,7-dichlorokynurenic acid, a potent antagonist at the N-methyl-D-aspartate receptor-associated glycine binding site. Mol Pharmacol 38:554-61 (1990). Read more (PubMed: 2172769) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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