Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (Alexa Fluor® 488) (ab201999)

Overview

  • Product name
    Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (Alexa Fluor® 488)
    See all 58K Golgi protein primary antibodies
  • Description
    Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker (Alexa Fluor® 488)
  • Host species
    Mouse
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Hamster, Cow, Dog, Pig, Monkey, African green monkey
  • Positive control
    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

Properties

Applications

Our Abpromise guarantee covers the use of ab201999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/2000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function
    Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • Pathway
    Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease
    Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • Sequence similarities
    In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab201999 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab201999, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab201999 staining 58K Golgi protein in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201999 at 1/2000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

References

ab201999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Chinese Hamster Ovary)
Permeabilization
Yes - 0.1 % Triton/PBS for 5 min @ RT
Specification
Chinese Hamster Ovary
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Sep 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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