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Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

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Overview

Product name

Anti-58K Golgi protein antibody [58K-9] - Golgi Marker
See all 58K Golgi protein products (7) ...

Description

Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker

Specificity

This product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.

Tested applications

WB, Functional Studies, ICC/IFmore details

Cross reactivity

Reacts with

Rat, Hamster, Dog, Human, Monkey, African Green Monkey

Predicted to work with

Cow, Pig

Immunogen

Full length native protein (purified) (Rat liver).

Positive control

Rat Liver. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

58K-9

Isotype

IgG1

Research areas

Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Signal Transduction >> Metabolism >> Amino Acids

  • Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] (ab27043)Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] (ab27043) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi.

Pathway

Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.

Involvement in disease

Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Sequence similarities

In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family.

Cellular localization

Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.

Target information above from: UniProt accessionO95954 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Formimidoyltetrahydrofolate cyclodeaminase antibody
  • Formimidoyltransferase cyclodeaminase antibody
  • Formiminotetrahydrofolate cyclodeaminase antibody
  • Formiminotransferase cyclodeaminase antibody
  • Formiminotransferase cyclodeaminase antibody
  • Formiminotransferase-cyclodeaminase antibody
  • FTCD antibody
  • FTCD antibody
  • FTCD_HUMAN antibody
  • Glutamate formiminotransferase antibody
  • Glutamate formiminotransferase antibody
  • Glutamate formyltransferase antibody
  • LCHC 1 antibody
  • LCHC1 antibody
  • LCHC1 antibody
see all

Database links

see all

Anti-58K Golgi protein antibody [58K-9] - Golgi Marker images:

  Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml

Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 58 kDa


Exposure time : 3 minutes

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).

Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

  Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] (ab27043)

Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] (ab27043)

ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

References for Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

This product has been referenced in:

  • Carvalho Get al. Participation of the cell polarity protein PALS1 to T-cell receptor-mediated NF-?B activation. PLoS One 6:e18159 (2011). ICC/IF; Human.Read more (PubMed: 21479189) »
  • Clauss Aet al. Overexpression of elafin in ovarian carcinoma is driven by genomic gains and activation of the nuclear factor kappaB pathway and is associated with poor overall survival. Neoplasia 12:161-72 (2010).Read more (PubMed: 20126474) »

See all 9 publications for this product

Publishing research using ab27043? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"