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Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
![Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)](/ps/datasheet/images/ACF1B58.jpg)
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Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
![Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)](/ps/datasheet/images/ab6284_3.jpg)
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)](/ps/datasheet/images/ab6284_6.jpg)
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Product Name
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58K Golgi protein antibody [58K-9] - Golgi Marker
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See all 58K Golgi protein antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
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Immunogen
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Full length native protein (purified) (Rat liver).
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Reacts with
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Hu, Rat, Cow, Dog, Hm, Mk
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Predicted to react
(species key)
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Pig
(Due to sequence homology)
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Specificity
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The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein.
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Tested applications
(see key)
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FuncS, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
FuncS: Use at an assay dependent dilution.
ICC/IF: 1/50 - 1/250.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells
For immunoblotting (colorimetric): whole rat liver extract
Antigen M.W.: 58 kDa
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Cellular localization
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Golgi Apparatus
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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58K Golgi protein antibodies are excellent for use as markers for the Golgi complex. The 58K Golgi protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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58K-9
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Ascitic fluid with 15mM sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product is in ascites form. Purified 58K-9 antibody is now available as ab27043.
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Form
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Liquid
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Concentration
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Batch dependent within range: 3.60 - 4.10 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 58K Golgi protein antibody [58K-9] - Golgi Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 58K Golgi protein antibody [58K-9] - Golgi Marker images, references, products related to ab6284 and other tools.
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58K Golgi protein antibody [58K-9] - Golgi Marker images:
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 Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
HeLa cells fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. Stained with anti-58K Golgi protein monoclonal antibody (1:50)followed by anti-Mouse IgG conjugated to Cy3 (1:100). This picture was kindly supplied as part of the review submitted by Sarah Coleman.
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Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
Cells were grown on coverslips, fixed with 70% methanol, and permeabilized with 0.5% Triton X-100 then double labeled by incubation with a rabbit polyclonal antibody against HE4 (a secreted ovarian cancer biomarker) and monoclonal antibodies against the 58K Golgi protein formiminotransferase cyclodeaminase (1:150) in PBS with 5% goat serum. After washing, appropriate species-specific, fluorochrome-conjugated secondary antibodies were applied.
This image was kindly supplied as part of the review submitted by Ronny Drapkin.
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
Formalin-fixed parafin embedded colon was incubated with ab6284 at range of dilutions (1/100 - 1/3000). Excellent staining of the colonic epithelium was seen using 1/1000 dilution. Heat-induced epitope retrieval was used. Ron Drapkin, Dana-Farber Cancer Institute
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)](/ps/datasheet/images/ab6284_7.jpg)
Formalin-fixed parafin embedded ovary was incubated with ab6284 at range of dilutions (1/100 - 1/3000). Excellent staining of the Golgi in Ovarian inclusion cysts was seen using 1/1000 dilution. Heat-induced epitope retrieval was used. Ron Drapkin, Dana-Farber Cancer Institute
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Immunohistochemistry - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
![Immunohistochemistry - 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)](/ps/datasheet/images/ab6284_8.jpg)
Ab6284 positively staining formaldehyde HEK 293 at 1/200. Used in conjunction with goat anti mouse (Alexa 546)1/1500. Nuclear staining achieved with Hoechst.
This image is courtesy of an Abreview submitted by Kun Lui on 19 September 2005. We do not have any further information relating to this image.
See Abreview
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References for 58K Golgi protein antibody [58K-9] - Golgi Marker (ab6284)
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This product has been used in: (two most recent references)
Kenny AV et al. The integrity of the glycine co-agonist binding site of N-methyl-D-aspartate receptors is a functional quality control checkpoint for cell surface delivery. J Biol Chem 284:324-33 (2009). ICC/IF; African Green Monkey. PubMed: 18990687
Turner NA et al. Endothelial cell ADAMTS-13 and VWF: production, release, and VWF string cleavage. Blood 114:5102-11 (2009). ICC/IF; Human. PubMed: 19822897
See all 21 publication references for this product.
If you publish research using ab6284 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 58K Golgi protein
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58K Golgi protein antibody [58K-9] - Golgi Marker - more information
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58K Golgi protein antibody [58K-9] - Golgi Marker - related products:
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58K Golgi protein antibody [58K-9] - Golgi Marker - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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