Products:Signal Transduction >> Metabolism >> Amino Acids
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ab5820 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lanes 1 - 2 : Anti-58K Golgi protein antibody - Golgi Marker (ab5820) at 1 µg/ml (Blocked with 5% BSA)
Lanes 3 - 4 : Anti-58K Golgi protein antibody - Golgi Marker (ab5820) at 1 µg/ml (Blocked with 3% Milk)
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 :
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 58 kDa
Exposure time : 30 seconds
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
SKOV3 cells (Ovarian cancer) were fixed with 70% methanol/30% acetone and incubated with ab5820 at 1/500 dilution (red). The staining shows good perinuclear distribution as expected for the Golgi. The DNA is stained with DAPI (blue). Dilutions of 1/250 - 1/500 are recommended for ab5820.
Ron Drapkin, Dana-Farber Cancer Institute
CaOV3 cells (Ovarian cancer) were fixed with 70% methanol/30% acetone and incubated with ab5820 at 1/500 dilution (red). The staining shows good perinuclear distribution as expected for the Golgi. The DNA is stained with DAPI (blue). Dilutions of 1/250 - 1/500 are recommended for ab5820.
Ron Drapkin, Dana-Farber Cancer Institute
All lanes : Anti-58K Golgi protein antibody - Golgi Marker (ab5820) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 58 kDa
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