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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-58K Golgi protein antibody - Golgi Marker
See all 58K Golgi protein products (7) ...
Description
Rabbit polyclonal to 58K Golgi protein - Golgi Marker
Specificity
This antibody detects a strong band at 58kDa band in human liver lysate and a weak band at 58kDa in both mouse and rat liver lysates. In each case the band is quenched by the immunising peptide.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human 58K Golgi protein.
(Peptide available as ab13733.)
Positive control
ab5820 gave a positive result in the following Tissue Lysates Human Liver Mouse Liver Rat Liver
General notes
lot # 297504 has a conc. of 0.4mg/ml. the label is corrected. SMB
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
Two stars (2 Abreviews)
Application notes
ICC/IF: 1/250 - 1/500. Fix with 70% methanol/30% acetone.
WB: 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Can be blocked with 58K Golgi protein peptide (ab13733).
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi.
Pathway
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
Involvement in disease
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
Sequence similarities
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family.
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
Target information above from: UniProt accessionO95954
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
Database links
- Entrez Gene: 10841 Human
- Entrez Gene: 14317 Mouse
- Entrez Gene: 397517 Pig
- Entrez Gene: 89833 Rat
- Omim: 606806 Human
- SwissProt: O95954 Human
- SwissProt: Q91XD4 Mouse
- SwissProt: P53603 Pig
see all
Anti-58K Golgi protein antibody - Golgi Marker images:
- 58K Golgi protein antibody - Golgi Marker (ab5820)
Western blot on human liver lysate (ab7919) using ab5820.
Lanes 1 & 2: ab5820 at 1/1000.
Lanes 3 & 4: ab5820 at 1/1000 + 1 g/ml blocking peptide.
Exposure time: 10 secs.
The lower non-specific band will probably disappear when using the antibody at higher dilutions.
Immunofluorescence - 58K Golgi protein antibody - Golgi Marker (ab5820)
SKOV3 cells (Ovarian cancer) were fixed with 70% methanol/30% acetone and incubated with ab5820 at 1/500 dilution (red). The staining shows good perinuclear distribution as expected for the Golgi. The DNA is stained with DAPI (blue). Dilutions of 1/250 - 1/500 are recommended for ab5820.
Ron Drapkin, Dana-Farber Cancer Institute
- 58K Golgi protein antibody - Golgi Marker (ab5820)
CaOV3 cells (Ovarian cancer) were fixed with 70% methanol/30% acetone and incubated with ab5820 at 1/500 dilution (red). The staining shows good perinuclear distribution as expected for the Golgi. The DNA is stained with DAPI (blue). Dilutions of 1/250 - 1/500 are recommended for ab5820.
Ron Drapkin, Dana-Farber Cancer Institute
Western blot - 58K Golgi protein antibody - Golgi Marker (ab5820)
All lanes : Anti-58K Golgi protein antibody - Golgi Marker (ab5820) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 58 kDa
References for Anti-58K Golgi protein antibody - Golgi Marker (ab5820)
This product has been referenced in:
- Liao Get al. An RNA-zipcode-independent mechanism that localizes Dia1 mRNA to the perinuclear ER through interactions between Dia1 nascent peptide and Rho-GTP. J Cell Sci 124:589-99 (2011). WB; Human.Read more (PubMed: 21266463) »
- Stuart CAet al. Insulin-stimulated translocation of glucose transporter (GLUT) 12 parallels that of GLUT4 in normal muscle. J Clin Endocrinol Metab 94:3535-42 (2009). WB; Human.Read more (PubMed: 19549745) »
See all 4 publications for this product
Publishing research using ab5820? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"