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Full length native protein (purified) (Rat liver).
Our Abpromise guarantee covers the use of ab27043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|Functional Studies||Use at an assay dependent dilution.|
|Flow Cyt||Use 2µg for 106 cells.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details.|
ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"