Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

Overview

  • Product nameAnti-58K Golgi protein antibody [58K-9] - Golgi Marker
    See all 58K Golgi protein primary antibodies
  • Description
    Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
  • Tested applicationsWB, Functional Studies, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Hamster, Cow, Dog, Human, Monkey, African Green Monkey
    Predicted to work with: Pig
  • Immunogen

    Full length native protein (purified) (Rat liver).

  • Positive control
    • Rat Liver. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
  • General notes

    We can conjugate this antibody to FITC for you (please see ab150248 for details).

Properties

Applications

Our Abpromise guarantee covers the use of ab27043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use 2µg for 106 cells.
ICC/IF Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details.

Target

  • FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    • LCHC1 antibody
    see all

Anti-58K Golgi protein antibody [58K-9] - Golgi Marker images

  • All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
    Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa


    Exposure time : 3 minutes
  • ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).
  • ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

This product has been referenced in:
  • Gasimli L  et al. Bioengineering murine mastocytoma cells to produce anticoagulant heparin. Glycobiology 24:272-80 (2014). Read more (PubMed: 24326668) »
  • Gallego-Iradi C  et al. KCNC3(R420H), a K(+) channel mutation causative in spinocerebellar ataxia 13 displays aberrant intracellular trafficking. Neurobiol Dis 71:270-9 (2014). ICC/IF ; Monkey . Read more (PubMed: 25152487) »

See all 18 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample Mouse Cell (hepatocytes)
Specification hepatocytes
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes)
Specification Astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (FRhK-4 cells)
Specification FRhK-4 cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (MDBK)
Loading amount 10 µg
Specification MDBK
Gel Running Conditions Reduced Denaturing (10% tris-glycine gel)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2012

Muchas gracias por su respuesta.

Lamento mucho que las sugerencias enviadas no ayudaran a mejorar los resultados.

Siendo que el anticuerpo está aun en garantía, le puedo ofrecer las siguientes opciones:

Manda...

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¡No hay de qué!

¡No dudes en contactarnos en castellano siempre que lo necesitéis!

Espero que las sugerencias puedan ayudar al cliente. En caso contrario, por favor, no dudes en contactarnos de nuevo.
...

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Te agradezco muchísimo que nos hayas enviado los detalles relativos al protocolo seguido con el anticuerpo, y lamento las molestias que el fallo del mismo pueda causarles.

Después de estudiar el protocolo enviado, me gustar...

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Thank you for confirming the additional information.


We haven't yet tested this antibody with mouse, so we are unsure how or if it will work in this species. There is a good chance for cross-reactivity since it works in rat, but we have...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SK-N-SH)
Specification SK-N-SH
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 02 2012

Thank you for your inquiry. Unfortunately, we do not have antibodies against 58K Golgi protein and GM130 available in our catalog that can be used for Yeast. I would like to recommend checking the Biocompare website which has an excellent antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"