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Full length native protein (purified) (Rat liver).
We can conjugate this antibody to FITC for you (please see ab150248 for details).
Our Abpromise guarantee covers the use of ab27043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|Functional Studies||Use at an assay dependent concentration.|
|Flow Cyt||Use 2µg for 106 cells. Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details.|
ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"