Overview

  • Product name
    Anti-58K Golgi protein antibody
    See all 58K Golgi protein primary antibodies
  • Description
    Goat polyclonal to 58K Golgi protein
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:

    SQLVECVPNFSEGKNQ

    , corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.

  • Positive control
    • Human liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 18315599

Target

  • Function
    Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • Pathway
    Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease
    Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • Sequence similarities
    In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Images

  • ab19072 at 3.8µg/ml staining 58K Golgi protein in human liver tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded tissue sections). Steamed antigen retrieval in citrate buffer pH 6 was performed. AP staining procedure was used for detection.

  • Anti-58K Golgi protein antibody (ab19072) at 0.05 µg/ml + Human liver lysate at 35 µg

    Predicted band size : 59 kDa

References

This product has been referenced in:
  • Perland E  et al. The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC Transporters Affected by Altered Nutrient Intake. J Mol Neurosci 61:199-214 (2017). Read more (PubMed: 27981419) »
  • Li H  et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). WB . Read more (PubMed: 26446566) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for send the question again. You can use the antibodies with the secondary, however be advised that we have only tested tissue sections and not cell cultures, though I would expect it is likely to work for cells as well. Please let me know if...

Read More

Unfortunately none of our Golgi markers have been tested against protists. Sorry I couldn't help you more,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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