Overview

  • Product nameAnti-58K Golgi protein antibody
    See all 58K Golgi protein primary antibodies
  • Description
    Goat polyclonal to 58K Golgi protein
  • Specificityab23932 is expected to recognise both reported isoforms.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Pig, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-LREQGRGKDQPGRL

    , corresponding to amino acids 203 - 216 of Human 58K Golgi protein.

  • Positive control
    • Human liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab23932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa). Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1).

Target

  • FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Anti-58K Golgi protein antibody images

  • Anti-58K Golgi protein antibody (ab23932) at 0.01 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab23932 staining 58K Golgi protein in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. AP staining was used as the detection method.
    Note the distinct staining of membranes and cytoplasm in a selection of hepatocytes.

References for Anti-58K Golgi protein antibody (ab23932)

This product has been referenced in:
  • Dixon BM  et al. Assessment of endoplasmic reticulum glutathione redox status is confounded by extensive ex vivo oxidation. Antioxid Redox Signal 10:963-72 (2008). Rat . Read more (PubMed: 18205546) »
  • Hagiwara H  et al. Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome. Histochem Cell Biol 126:251-9 (2006). Read more (PubMed: 16534631) »

See all 2 Publications for this product

Product Wall

Thank you for send the question again. You can use the antibodies with the secondary, however be advised that we have only tested tissue sections and not cell cultures, though I would expect it is likely to work for cells as well. Please let me know if...

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Thank you for contacting Abcam.

The antibody ab23932 should still be ok after 6 months at 4C, you may need to use a slightly higher concentration for this antibody, but it should be fine.

Please let me know if there is anything else...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"