Overview

  • Product name
    Anti-58K Golgi protein antibody
    See all 58K Golgi protein primary antibodies
  • Description
    Goat polyclonal to 58K Golgi protein
  • Specificity
    ab23932 is expected to recognise both reported isoforms.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Pig, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-LREQGRGKDQPGRL

    , corresponding to amino acids 203 - 216 of Human 58K Golgi protein.

  • Positive control
    • Human liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab23932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa). Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1).

Target

  • Function
    Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • Pathway
    Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease
    Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • Sequence similarities
    In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Images

  • Anti-58K Golgi protein antibody (ab23932) at 0.01 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab23932 staining 58K Golgi protein in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. AP staining was used as the detection method.
    Note the distinct staining of membranes and cytoplasm in a selection of hepatocytes.

References

This product has been referenced in:
  • Gasimli L  et al. Bioengineering murine mastocytoma cells to produce anticoagulant heparin. Glycobiology 24:272-80 (2014). Read more (PubMed: 24326668) »
  • Dixon BM  et al. Assessment of endoplasmic reticulum glutathione redox status is confounded by extensive ex vivo oxidation. Antioxid Redox Signal 10:963-72 (2008). Rat . Read more (PubMed: 18205546) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for send the question again. You can use the antibodies with the secondary, however be advised that we have only tested tissue sections and not cell cultures, though I would expect it is likely to work for cells as well. Please let me know if...

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Thank you for contacting Abcam.

The antibody ab23932 should still be ok after 6 months at 4C, you may need to use a slightly higher concentration for this antibody, but it should be fine.

Please let me know if there is anything else...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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