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Anti-5HT1A Receptor antibody (ab44635) at 1/500 dilution +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Exposure time : 16 minutes
5HT1A Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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