You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-5HT1A Receptor antibody (ab44635)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-5HT1A Receptor antibody
See all 5HT1A Receptor products (8) ...

Description

Rabbit polyclonal to 5HT1A Receptor

Tested applications

WB, ICC/IF, Othermore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide: EVHRVGNSKEHLPLPSESG , corresponding to amino acids 294-312 of Rat 5HT1A Receptor

EVHRVGNSKE HLPLPSESG

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Rat cortex, arcuate and hippocampus

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> G protein signaling >> GPCR
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Serotonin Receptors

  • Western blot - 5HT1A Receptor antibody (ab44635)Western blot - 5HT1A Receptor antibody (ab44635) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab44635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/100Detects a band ...Read more →

    WB: 1/100Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).(We recommend overnight incubation at 4C. )

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution.

  • ShowHide

    Other

     Other: Use at an assay d...Read more →

    Other: Use at an assay dependent dilution. (IHC-FrFl (vibratome-sectioned free floating tissue))

Application notes

Is unsuitable for or IHC-P.

Target

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP08908 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5 HT1A receptor antibody
  • 5 HT 1A antibody
  • 5 HT receptor 1A antibody
  • 5 HT1A antibody
  • 5 hydroxytryptamine (serotonin) receptor 1A antibody
  • 5 hydroxytryptamine receptor 1A antibody
  • 5-HT-1A antibody
  • 5-HT1A antibody
  • 5-hydroxytryptamine receptor 1A antibody
  • 5HT 1A antibody
  • 5HT 1A receptor antibody
  • 5HT receptor 1A antibody
  • 5HT1A_HUMAN antibody
  • ADRB2RL1 antibody
  • ADRBRL1 antibody
  • G 21 antibody
  • G protein coupled receptor antibody
  • G-21 antibody
  • Gpcr 18 antibody
  • Gpcr18 antibody
  • Guanine nucleotide binding regulatory protein-coupled receptor antibody
  • HTR1A antibody
  • Serotonin 1A receptor antibody
  • Serotonin 5HT1A receptor antibody
  • Serotonin receptor 1A antibody
see all

Database links

see all

Anti-5HT1A Receptor antibody images:

  Western blot - 5HT1A Receptor antibody (ab44635)

Western blot - 5HT1A Receptor antibody (ab44635)

Anti-5HT1A Receptor antibody (ab44635) at 1/500 dilution + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 47 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)


Exposure time : 16 minutes

5HT1A Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References for Anti-5HT1A Receptor antibody (ab44635)

This product has been referenced in:

  • Rozeske RRet al. Uncontrollable, but not controllable, stress desensitizes 5-HT1A receptors in the dorsal raphe nucleus. J Neurosci 31:14107-15 (2011).Read more (PubMed: 21976495) »
  • Lehner Met al. Colocalisation of c-Fos and glucocorticoid receptor as well as of 5-HT(1A) and glucocorticoid receptor immunoreactivity-expressing cells in the brain structures of low and high anxiety rats. Behav Brain Res 200:150-9 (2009).Read more (PubMed: 19373980) »

See all 3 publications for this product

Publishing research using ab44635? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"