Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Serotonin Receptors
Overview
Product name
Anti-5HT1A Receptor antibody
See all 5HT1A Receptor products (8) ...
Description
Rabbit polyclonal to 5HT1A Receptor
Specificity
ab64994 detects endogenous levels of total 5HT1A Receptor.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from internal sequence of human 5HT1A Receptor.
Positive control
Extracts from HepG2 cells and Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab64994 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> GPCR
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Serotonin Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08908
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 HT1A receptor antibody
- 5 HT 1A antibody
- 5 HT receptor 1A antibody
see all
Database links
- Entrez Gene: 3350 Human
- Entrez Gene: 15550 Mouse
- Entrez Gene: 24473 Rat
- Omim: 109760 Human
- SwissProt: P08908 Human
- SwissProt: Q64264 Mouse
- SwissProt: P19327 Rat
- Unigene: 247940 Human
see all
Anti-5HT1A Receptor antibody images:
Western blot - 5HT1A Receptor antibody (ab64994)
All lanes : Anti-5HT1A Receptor antibody (ab64994) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunising peptide
Lane 2 : Extracts from Jurkat cells with no immunising peptide
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
Western blot - 5HT1A Receptor antibody (ab64994)
All lanes : Anti-5HT1A Receptor antibody (ab64994) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 52 kDa,85 kDa. We are unsure as to the identity of these extra bands.
References for Anti-5HT1A Receptor antibody (ab64994)
ab64994 has not yet been referenced specifically in any publications.
Publishing research using ab64994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"