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Western blot - 5HT1A Receptor antibody (ab64994)
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Western blot - 5HT1A Receptor antibody (ab64994)
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Product Name
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5HT1A Receptor antibody
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See all 5HT1A Receptor antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to 5HT1A Receptor
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Immunogen
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Synthetic peptide derived from internal sequence of human 5HT1A Receptor.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab64994 detects endogenous levels of total 5HT1A Receptor.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HepG2 cells and Jurkat cells.
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Cellular localization
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Membrane; multi-pass membrane protein.
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> GPCR
Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Serotonin Receptors
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Relevance
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5-HT receptors are receptors for the neurotransmitter and peripheral signal mediator 5-hydroxytryptamine (5-HT), also known as serotonin. 5-HT receptors are of great interest as they also mediate the effects of a broad range of pharmaceutical and hallucinogenic drugs. Like most other members of this family, the 5HT1A receptor (also referred to as 5HT receptor 1A or serotonin receptor 1A) is a G-protein coupled receptor. It acts by decreasing cellular levels of cAMP and causes neuronal inhibition. In mammals, 5HT1A receptor expression is strongest in CNS limbic pathways that control emotion, and 5HT1A receptor function is associated with a number of behavioural effects (sleep, feeding, thermoregulation, aggression and anxiety). Agonists for the 5HT1A receptor include LSD, buspirone, and psilocin.
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5HT1A Receptor antibody (ab64994) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab64994 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 5HT1A Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 5HT1A Receptor antibody images, references, products related to ab64994 and other tools.
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5HT1A Receptor antibody images:
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 Western blot - 5HT1A Receptor antibody (ab64994)
All lanes : 5HT1A Receptor antibody (ab64994) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunising peptide
Lane 2 : Extracts from Jurkat cells with no immunising peptide
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
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Western blot - 5HT1A Receptor antibody (ab64994)
All lanes : 5HT1A Receptor antibody (ab64994) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 52 kDa,85 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to 5HT1A Receptor
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5HT1A Receptor antibody - more information
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Customer reviews (feedback) regarding 5HT1A Receptor antibody |
Customer FAQs regarding 5HT1A Receptor antibody |
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5HT1A Receptor antibody - related products:
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5HT1A Receptor antibody - other tools:
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Contact Abcam with a Technical Enquiry about 5HT1A Receptor antibody |
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