Anti-5HT1A Receptor antibody (ab85615)

Overview

  • Product nameAnti-5HT1A Receptor antibodySee all 5HT1A Receptor primary antibodies ...
  • Description
    Rabbit polyclonal to 5HT1A Receptor
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat 5HT1A Receptor.

    (Peptide available as ab98259.)

  • Positive control
    • This antibody gave a positive signal in both Mouse and Rat hippocampus tissue lysate as well as the following whole cell lysates: Caco2; HepG2; Jurkat. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab85615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 46 kDa).Can be blocked with Rat 5HT1A Receptor peptide (ab98259).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 1A antibody
    • 5 HT receptor 1A antibody
    • 5 HT1A antibody
    • 5 HT1A receptor antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled antibody
    • 5 hydroxytryptamine receptor 1A antibody
    • 5-HT-1A antibody
    • 5-HT1A antibody
    • 5-hydroxytryptamine receptor 1A antibody
    • 5HT 1A antibody
    • 5HT 1A receptor antibody
    • 5HT receptor 1A antibody
    • 5HT1a antibody
    • 5HT1A_HUMAN antibody
    • ADRB2RL1 antibody
    • ADRBRL1 antibody
    • G 21 antibody
    • G protein coupled receptor antibody
    • G-21 antibody
    • Gpcr 18 antibody
    • Gpcr18 antibody
    • Guanine nucleotide binding regulatory protein-coupled receptor antibody
    • HTR1A antibody
    • PFMCD antibody
    • Serotonin 1A receptor antibody
    • Serotonin 5HT1A receptor antibody
    • Serotonin receptor 1A antibody
    see all

Anti-5HT1A Receptor antibody images

  • All lanes : Anti-5HT1A Receptor antibody (ab85615) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab85615 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of 5HT1A Receptor staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85615, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-5HT1A Receptor antibody (ab85615)

ab85615 has not yet been referenced specifically in any publications.

Product Wall

I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only t...

Read More
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

Submitted Jul 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"