Overview

  • Product name
    Anti-5HT1A Receptor antibody
    See all 5HT1A Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT1A Receptor
  • Tested applications
    Suitable for: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Rat 5HT1A Receptor aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: P19327
    (Peptide available as ab98259)

  • Positive control
    • This antibody gave a positive signal in both Mouse and Rat hippocampus tissue lysate as well as the following whole cell lysates: Caco2; HepG2; Jurkat. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab85615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 26076134
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 46 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 1A antibody
    • 5 HT receptor 1A antibody
    • 5 HT1A antibody
    • 5 HT1A receptor antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled antibody
    • 5 hydroxytryptamine receptor 1A antibody
    • 5-HT-1A antibody
    • 5-HT1A antibody
    • 5-hydroxytryptamine receptor 1A antibody
    • 5HT 1A antibody
    • 5HT 1A receptor antibody
    • 5HT receptor 1A antibody
    • 5HT1a antibody
    • 5HT1A_HUMAN antibody
    • ADRB2RL1 antibody
    • ADRBRL1 antibody
    • G 21 antibody
    • G protein coupled receptor antibody
    • G-21 antibody
    • Gpcr 18 antibody
    • Gpcr18 antibody
    • Guanine nucleotide binding regulatory protein-coupled receptor antibody
    • Htr1a antibody
    • PFMCD antibody
    • Serotonin 1A receptor antibody
    • Serotonin 5HT1A receptor antibody
    • Serotonin receptor 1A antibody
    see all

Images

  • IHC image of 5HT1A Receptor staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85615, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab85615 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of rat hippocampus labelling 5HT1A with ab85615 (red). For immunofluorescence analysis, the rats were anaesthetized using chloral hydrate and then were perfused with 150 mL of saline solution and 150–200 mL of 4% paraformaldehyde in 0.1 M phosphate buffer (PB). Then, the brains of the rats were isolated and fixed in 4% paraformaldehyde for 2–3 h. The rat brain sections were cut on a freezing microtome at a thickness of 16 μm and were washed with phosphate-buffered saline (PBS) and pre-incubated in 5% horse serum. Subsequently, the sections were incubated with Anti-5HT1A Receptor antibody (ab85615) for 24 h at 4°C, washed with PBS and incubated with the secondary antibody and neuro tracer for 2 h at room temperature while protected from light. The sections were examined on a Nikon C2 plus confocal laser-scanning microscope.

  • All lanes : Anti-5HT1A Receptor antibody (ab85615) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 secondsThe expression profile observed is consistent with what has been described in the literature (PMID: 16772521).

References

This product has been referenced in:
  • Savignac HM  et al. Prebiotic administration normalizes lipopolysaccharide (LPS)-induced anxiety and cortical 5-HT2A receptor and IL1-ß levels in male mice. Brain Behav Immun 52:120-31 (2016). WB . Read more (PubMed: 26476141) »
  • Zheng Y  et al. In silico Analysis and Experimental Validation of Lignan Extracts from Kadsura longipedunculata for Potential 5-HT1AR Agonists. PLoS One 10:e0130055 (2015). Read more (PubMed: 26076134) »

See all 2 Publications for this product

Customer reviews and Q&As

I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only t...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jul 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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