Overview

  • Product nameAnti-5HT1B Receptor antibodySee all 5HT1B Receptor primary antibodies ...
  • Description
    Rabbit polyclonal to 5HT1B Receptor
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides, corresponding to amino acids 8-26 (CAPPPPAGSETWVPQANLS) and 263-278 (STSSVTSINSRVPDVP) of 5HT1B Receptor.

  • Positive control
    • Tested with human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
    Palmitoylated.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P28222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 5 HT1DB antibody
    • 5 hydroxytryptamine (serotonin) receptor 1B antibody
    • 5 hydroxytryptamine receptor 1B antibody
    • 5-HT-1B antibody
    • 5-HT-1D-beta antibody
    • 5-HT1B antibody
    • 5-hydroxytryptamine receptor 1B antibody
    • 5HT1B_HUMAN antibody
    • HTR1B antibody
    • HTR1D2 antibody
    • HTR1DB antibody
    • S12 antibody
    • Serotonin 1D beta receptor antibody
    • Serotonin receptor 1B antibody
    see all

Anti-5HT1B Receptor antibody images

  • ab17976 staining 5HT1B in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBFand blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview



  • Predicted band size : 47 kDa
    Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected. Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected.
  • ab2607 (4µg/ml) staining 5HT1B in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane compartment of the mature seminiferous tubules and possible interstitial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-5HT1B Receptor antibody (ab13896)

This product has been referenced in:
  • Ahnstedt H  et al. Male-female differences in upregulation of vasoconstrictor responses in human cerebral arteries. PLoS One 8:e62698 (2013). IHC-P ; Human . Read more (PubMed: 23658641) »
  • Davis RP  et al. 5-hydroxytryptamine (5-HT) reduces total peripheral resistance during chronic infusion: direct arterial mesenteric relaxation is not involved. BMC Pharmacol 12:4 (2012). WB . Read more (PubMed: 22559843) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative 10% NBF
Username

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Verified customer

Submitted Dec 16 2013

Thank you for your response.

The information provided in the two e-mails is very limited. In the Lab, this antibody has been tested and characterized for Western blot application on human brain tissue lysate and not on SHySy5 cells. You may n...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting us.
The immunogens are not commercially available at this time.
Please let us know if you have any other questions.

Thanks for your reply.

It is good to know that you have used the Trizol extraction method successfully with other samples. The reason I suggested the negative control sample was because it would, if there is an issue with the antibody specifi...

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Thanks again for submitting your protocol details as well as the western blotting image.

I just have a couple additional questions:

1) Have you run a negative control sample that you expect would not contain 5HT1B?

2) Have...

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Thank you for your enquiry. I am sorry to hear this vial of ab13896 is giving you trouble. I am currently reviewing the information you have generously provided and will follow up with you as soon as possible.

Thanks for your patience!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"