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Synthetic peptides, corresponding to amino acids 8-26 (CAPPPPAGSETWVPQANLS) and 263-278 (STSSVTSINSRVPDVP) of 5HT1B Receptor.
Our Abpromise guarantee covers the use of ab13896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IF||Use at an assay dependent concentration. PubMed: 24642693|
ab17976 staining 5HT1B in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBFand blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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