Overview
- Product nameAnti-5HT1F Receptor antibodySee all 5HT1F Receptor primary antibodies ...
- DescriptionRabbit polyclonal to 5HT1F Receptor
- Specificityab28695 recognises hydroxytryptamine 1F receptor (HTR1F).
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, Chimpanzee - Immunogen
A region within a synthetic peptide: MDFLNSSDQN LTSEELLNRM PSKILVSLTL SGLALMTTTI NSLVIAAIIV, corresponding to N terminal amino acids 1-50 of Human 5HT1F Receptor
- Positive controlFetal liver lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab28695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| ELISA | ELISA: 1/62500. |
Target
- Relevance5HT1F is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. 5HT1F is a member of the GPCR family (subfamily: Serotonin). Expression of 5HT1F has been reported in various brain structures, including cortex, hippocampus, trigeminal ganglia, and cerebral blood vessels. Expression in peripheral tissues is limited to uterus, mesentery, and artery. No expression has been detected in heart, kidney, liver, pancreas, or spleen.
- Cellular localizationCell membrane; Multi pass membrane protein.
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Database links
- Entrez Gene: 3355 Human
- Entrez Gene: 3355 Human
- Entrez Gene: 3355 Human
- Entrez Gene: 15557 Mouse
- Entrez Gene: 60448 Rat
- Omim: 182134 Human
- SwissProt: P30939 Human
- SwissProt: P30939 Human
- SwissProt: P30939 Human
- SwissProt: Q02284 Mouse
- SwissProt: P30940 Rat
- Unigene: 248136 Human
- Unigene: 44301 Rat
see all
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Alternative names
- 5 HT 1F antibody5 HT1F antibody5 hydroxytryptamine (serotonin) receptor 1F antibody
- 5 hydroxytryptamine 1F receptor antibody5 hydroxytryptamine receptor 1E beta antibody5 Hydroxytryptamine Receptor 1F antibody5 Hydroxytryptamine Serotonin Receptor 1E Beta antibody5 Hydroxytryptamine Serotonin Receptor 1F antibody5HT 1F antibody5HT1F antibodyHGNC5292 antibodyHTR 1F antibodyHTR1EB antibodyHTR1EL antibodyHTR1EL antibodyHTR1F antibodyMR77 antibodyMR77 antibodySerotonin receptor 1E beta antibodySerotonin Receptor Gene antibody
see all
Anti-5HT1F Receptor antibody images
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Western blot - 5HT1F Receptor antibody (ab28695)Anti-5HT1F Receptor antibody (ab28695) at 2.5 µg/ml + Fetal liver lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 44 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
References for Anti-5HT1F Receptor antibody (ab28695)
ab28695 has not yet been referenced specifically in any publications.
