Overview

  • Product nameAnti-5HT3A receptor antibodySee all 5HT3A receptor primary antibodies ...
  • Description
    Rabbit polyclonal to 5HT3A receptor
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides, corresponding to amino acids 21-36 and 421-436 of Rat 5HT3 Receptor.

  • Positive control
    • human brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab13897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificityExpressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3B antibody
    • 5 HT3R antibody
    • 5 HT3RA antibody
    • 5 HTR3B antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5 Hydroxytryptamine 3 receptor B subunit antibody
    • 5 Hydroxytryptamine receptor 3 antibody
    • 5 Hydroxytryptamine receptor 3 subunit B antibody
    • 5 Hydroxytryptamine receptor 3B antibody
    • 5 Hydroxytryptamine serotonin receptor 3B antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT 3 antibody
    • 5HT 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR 3 antibody
    • HTR3 antibody
    • Htr3a antibody
    • HTR3B antibody
    • Serotonin gated ion channel receptor antibody
    • Serotonin receptor 3 antibody
    • Serotonin receptor 3A antibody
    • Serotonin receptor 3B antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Anti-5HT3A receptor antibody images

  • All lanes : Anti-5HT3A receptor antibody (ab13897) at 3 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Mouse Brain tissue lysate
    Lane 3 : Rat Brain tissue lysate


    Predicted band size : 55 kDa
  • IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-5HT3A receptor antibody (ab13897)

This product has been referenced in:
  • Nguyen H  et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . Read more (PubMed: 17919312) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Mouse Tissue sections (tongue, taste buds)
Specification tongue, taste buds
Permeabilization Yes - 0.3 % Triton, and 1 % Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 02 2014

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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Thank you for contacting Abcam.

The amino acids correspond to the protein sequence with the signal peptide (SwissProt: P35563). We normally look for at least 85% degree of homology before we would recommend using an antibody in an untested sp...

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Thank you for contacting us. The detection kit we used for the IHC image is the Leica Bond refine polymer detection kit (for the automated Leica Bond IHC system). The system uses small linkers to enhance and refine the detection of the target p...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13897. To check the status of the order please contact our C...

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Application Western blot
Sample Rat Cell lysate - whole cell (rat cerebellar homogenate)
Loading amount 20 µg
Specification rat cerebellar homogenate
Treatment control and 20 mg/kg cocaine
Gel Running Conditions Reduced Denaturing
Blocking step Commercially available blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Mr. Jean-Bernard Dietrich

Verified customer

Submitted Sep 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"