Overview

  • Product name
    Anti-5HT3A receptor antibody
    See all 5HT3A receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT3A receptor
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat 5HT3A receptor. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
    Database link: P35563

  • Positive control
    • human brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab13897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificity
    Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • Cellular localization
    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Images

  • All lanes : Anti-5HT3A receptor antibody (ab13897) at 3 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Mouse Brain tissue lysate
    Lane 3 : Rat Brain tissue lysate


    Predicted band size : 55 kDa
  • IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Performed under reducing conditions.

    Predicted band size : 55 kDa

    Western Blot of human brain lysate staining 5HT3A receptor with ab13897

References

This product has been referenced in:
  • Nguyen H  et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . Read more (PubMed: 17919312) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - 0.1% TritonX-100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Weihua Wang​

Verified customer

Submitted Apr 07 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Cell (SH-SY5Y))
Permeabilization
Yes - TRITON X-100 (0'25%)
Specification
Human Cell (SH-SY5Y)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 06 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10% SDS-acryamide gel)
Sample
Rat Tissue lysate - whole (rat-colon)
Specification
rat-colon
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

Submitted Sep 22 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Mouse Tissue sections (tongue, taste buds)
Specification
tongue, taste buds
Permeabilization
Yes - 0.3 % Triton, and 1 % Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 02 2014

Thank you for contacting Abcam.

The amino acids correspond to the protein sequence with the signal peptide (SwissProt: P35563). We normally look for at least 85% degree of homology before we would recommend using an antibody in an untested sp...

Read More

Thank you for contacting us. The detection kit we used for the IHC image is the Leica Bond refine polymer detection kit (for the automated Leica Bond IHC system). The system uses small linkers to enhance and refine the detection of the target p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat cerebellar homogenate)
Loading amount
20 µg
Specification
rat cerebellar homogenate
Treatment
control and 20 mg/kg cocaine
Gel Running Conditions
Reduced Denaturing
Blocking step
Commercially available blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Mr. Jean-Bernard Dietrich

Verified customer

Submitted Sep 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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