Overview
- Product nameAnti-5T4 antibodySee all 5T4 primary antibodies ...
- DescriptionMouse monoclonal to 5T4
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Rat, Human
- Immunogen
Recombinant fragment, corresponding to amino acids 219-329 of Human 5T4, with a 26 KDa tag (NP_006661).
- Positive controlHeLa and PC12 cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2b
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab88091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- Tissue specificityExpressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
- Sequence similaritiesContains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 7162 Human
- Entrez Gene: 83684 Rat
- Omim: 190920 Human
- SwissProt: Q13641 Human
- SwissProt: Q5PQV5 Rat
- Unigene: 82128 Human
- Unigene: 43122 Rat
Target information above from: UniProt accession
Q13641
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 5T4 antibody5T4 oncofetal antigen antibody5T4 oncofetal trophoblast glycoprotein antibody
- 5T4 oncotrophoblast glycoprotein antibody5T4AG antibodyAW495680 antibodyM6P1 antibodyTPBG antibodyTPBG_HUMAN antibodyTrophoblast Glycoprotein antibody
see all
Anti-5T4 antibody images
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Western blot - 5T4 antibody (ab88091)Anti-5T4 antibody (ab88091) at 5 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 47 kDa
Observed band size : 47 kDa -
Western blot - 5T4 antibody (ab88091)Anti-5T4 antibody (ab88091) at 5 µg/ml + PC12 cell lysate at 50 µg
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?) -
Sandwich ELISA - 5T4 antibody (ab88091)Detection limit for ab88091 is approximately 1ng/ml as a capture antibody.
References for Anti-5T4 antibody (ab88091)
ab88091 has not yet been referenced specifically in any publications.
