Anti-5HT1A Receptor antibody (ab64994)


  • Product name
    Anti-5HT1A Receptor antibody
    See all 5HT1A Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT1A Receptor
  • Host species
  • Specificity
    ab64994 detects endogenous levels of total 5HT1A Receptor.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 5HT1A Receptor (internal sequence). Immunogen Range: 291-340
    Database link: P08908
    (Peptide available as ab133019)

  • Positive control
    • Extracts from HepG2 cells and Jurkat cells.



Our Abpromise guarantee covers the use of ab64994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.


  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 1A antibody
    • 5 HT receptor 1A antibody
    • 5 HT1A antibody
    • 5 HT1A receptor antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled antibody
    • 5 hydroxytryptamine receptor 1A antibody
    • 5-HT-1A antibody
    • 5-HT1A antibody
    • 5-hydroxytryptamine receptor 1A antibody
    • 5HT 1A antibody
    • 5HT 1A receptor antibody
    • 5HT receptor 1A antibody
    • 5HT1a antibody
    • 5HT1A_HUMAN antibody
    • ADRB2RL1 antibody
    • ADRBRL1 antibody
    • G 21 antibody
    • G protein coupled receptor antibody
    • G-21 antibody
    • Gpcr 18 antibody
    • Gpcr18 antibody
    • Guanine nucleotide binding regulatory protein-coupled receptor antibody
    • Htr1a antibody
    • PFMCD antibody
    • Serotonin 1A receptor antibody
    • Serotonin 5HT1A receptor antibody
    • Serotonin receptor 1A antibody
    see all


  • All lanes : Anti-5HT1A Receptor antibody (ab64994) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells with no immunising peptide
    Lane 2 : Extracts from Jurkat cells with no immunising peptide
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa
    Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-5HT1A Receptor antibody (ab64994) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)

    Predicted band size: 46 kDa
    Observed band size: 46 kDa
    Additional bands at: 52 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


ab64994 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12% gradient gel)
Rat Tissue lysate - other (Spinal cord, solubilized membranes)
Spinal cord, solubilized membranes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 30 2014

Vielen Dank für Ihren Anruf und für Ihre Geduld.

Es tut mir leid, dass Sie so lange auf eine Antwort warten mussten. Sie können das Peptide nun unter der Artikelnummer ab133019 bestellen.

Click here (or use the following: http://www.a...

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Ich möchte Sie hiermit wissen lassen, dass ich noch an Ihrer Anfrage arbeite. Ich habe mich mit dem Labor, welches das Peptid nun für Sie herstellt, wiederholt in Verbindung gesetzt, warte aber immer noch auf eine Antwort bezüglich des Zeitpunktes bzw....

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Vielen Dank für Ihren Anruf.

Ich habe gute Nachrichten für Sie: wir werden Ihnen in kürze das Blockpeptide anbieten können, da unser Zulieferer mit der Produktion begonnen hat. Leider kann ich noch keine Angaben zum Zeitpunkt, der Menge oder...

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