Recombinant Anti-S31 antibody [EPR10789(B)] (ab154813)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10789(B)] to S31
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-S31 antibody [EPR10789(B)]
See all S31 primary antibodies -
Description
Rabbit monoclonal [EPR10789(B)] to S31 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human S31 (intracellular).
Database link: P28566 -
Positive control
- Jurkat and SH-SY5Y cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10789(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
Target
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Relevance
This is one of the several different receptors for 5-HT, a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. The 5-HT1E receptor is expressed in human cortex, caudate, putamen, and amygdala, as well as in dorsal root ganglia and in coronary artery. ESTs have been isolated from human brain cancer libraries. -
Cellular localization
Cell Membrane, Multi-pass membrane protein. -
Database links
- Entrez Gene: 3354 Human
- Omim: 182132 Human
- SwissProt: P28566 Human
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Alternative names
- 5 HT1E antibody
- 5 hydroxytryptamine (serotonin) receptor 1E antibody
- 5 hydroxytryptamine (serotonin) receptor 1E G protein coupled antibody
see all
Images
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All lanes : Anti-S31 antibody [EPR10789(B)] (ab154813) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling S31 with ab154813 at 1/10 dilution (red), compared to a rabbit IgG negative control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154813 has not yet been referenced specifically in any publications.