Overview

  • Product nameAnti-5HT2A Receptor antibody
    See all 5HT2A Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT2A Receptor
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GDGPRLYHNDFNSRDANTSE

    , corresponding to N terminal amino acids 22-41 of Rat 5HT2A Receptor

  • Positive control
    • Rat cortex, amygdala and hippocampus tissue; mouse frontal cortical neuron cultures.
  • General notesUpon delivery dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.

Properties

Applications

Our Abpromise guarantee covers the use of ab66049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300 - 1/500. Biotin/Streptavidin-HRP Technique.
WB 1/100. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.
  • Cellular localizationCell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 2 antibody
    • 5 HT 2A antibody
    • 5 HT2 receptor antibody
    • 5 HT2A antibody
    • 5 hydroxytryptamine receptor 2A antibody
    • 5-HT-2 antibody
    • 5-HT-2A antibody
    • 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled antibody
    • 5-hydroxytryptamine 2A receptor antibody
    • 5-hydroxytryptamine receptor 2A antibody
    • 5HT2A_HUMAN antibody
    • HTR 2 antibody
    • HTR 2A antibody
    • HTR2 antibody
    • HTR2, formerly antibody
    • HTR2A antibody
    • serotonin 5-HT-2 receptor, formerly antibody
    • serotonin 5-HT-2A receptor antibody
    • Serotonin receptor 2A antibody
    see all

Anti-5HT2A Receptor antibody images

  • ab66049 at 1/100 dilution staining 5HT2A Receptor in Mouse frontal cortical neuron cultures (p1 pups, day 4 culture, for 48hrs) by Immunohistochemistry, Paraformaldehyde-fixed tissue.
  • ab66049, at 1/300 dilution staining 5HT2A Receptor in Rat cerebral cortex by Immunohistochemistry, Paraformaldehyde-fixed tissue.
  • Anti-5HT2A Receptor antibody (ab66049) at 1/400 dilution + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-5HT2A Receptor antibody (ab66049)

This product has been referenced in:
  • Hooper CW  et al. Selective serotonin reuptake inhibitor exposure constricts the mouse ductus arteriosus in utero. Am J Physiol Heart Circ Physiol 311:H572-81 (2016). Read more (PubMed: 27371685) »
  • Wei XY  et al. Expressions of 5-HT/5-HT(2A) receptors and phospho-protein kinase C theta in the pre-Bötzinger complex in normal and chronic intermittent hypoxic rats. Neuroscience 168:61-73 (2010). Electron Microscopy, IHC-FoFr ; Rat . Read more (PubMed: 20303395) »

See all 2 Publications for this product

Product Wall

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for contacting us.

Your credit note ID is 21936.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed ...

Read More

Thank you for your message and for providing this further information. I am sorry to hear these products did not work a stated on our datasheet. I appreciate the time you have spent on these experiments, and would like to offer a free of charge repl...

Read More

Thank you for contacting us.

I am very sorry to hear that you have been experiencing troubles when using our products. I would also like to thank you for providing me with details of you protocol.Our products are covered by Abpromise guara...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"