Anti-5HT2C Receptor antibody (ab32887)


  • Product nameAnti-5HT2C Receptor antibody
    See all 5HT2C Receptor primary antibodies
  • Description
    Goat polyclonal to 5HT2C Receptor
  • SpecificityAb32887 recognises 5HT2C Receptor.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 437-446 of Human 5HT2C Receptor.

  • Positive control
    • Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.



Our Abpromise guarantee covers the use of ab32887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
ELISA 1/64000.
IHC-Fr Use at an assay dependent concentration. Abreview 30686 suggests that this antibody is not suitable for this application


  • FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe PDZ domain-binding motif is involved in the interaction with MPDZ.
  • Post-translational
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 Hydroxytryptamine 2C receptor antibody
    • 5-HT-1C antibody
    • 5-ht-1c receptor antibody
    • 5-HT-2C antibody
    • 5-HT1C antibody
    • 5-HT2C antibody
    • 5-HTR2C antibody
    • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
    • 5-hydroxytryptamine receptor 1C antibody
    • 5-hydroxytryptamine receptor 2C antibody
    • 5HT1C antibody
    • 5HT2C antibody
    • 5HT2C_HUMAN antibody
    • 5HTR2C antibody
    • 5Hydroxytryptamine 2C receptor antibody
    • Htr1c antibody
    • HTR2C antibody
    • serotonin 1c receptor antibody
    • serotonin 2c receptor antibody
    • Serotonin 5-HT-2C receptor antibody
    • Serotonin receptor 2C antibody
    see all

Anti-5HT2C Receptor antibody images

  • Anti-5HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 hour

References for Anti-5HT2C Receptor antibody (ab32887)

ab32887 has not yet been referenced specifically in any publications.

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Great news! The blocking peptide has been added as 5HT2C Receptor peptide ab168550.

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Dec 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative formalin
Antigen retrieval step Enzymatic
Permeabilization Yes - 1xPBT
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C

Abcam user community

Verified customer

Submitted Sep 04 2012

I am very pleased to hear you would like to accept our offer and test ab32887 and ab44640 in IHC-FoFr and WB, respectively. These codes will give you: 1 free primary antibody each before the expiration date. To redeem this offer, please submit an ...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (hypothalamus PVN sections)
Specification hypothalamus PVN sections
Fixative ethanol 70%
Permeabilization Yes - triton-x
Blocking step 2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Ms. Rebecca Ring

Verified customer

Submitted Sep 30 2011