Anti-5HT2C Receptor antibody (ab32887)

Overview

  • Product name
    Anti-5HT2C Receptor antibody
    See all 5HT2C Receptor primary antibodies
  • Description
    Goat polyclonal to 5HT2C Receptor
  • Specificity
    Ab32887 recognises 5HT2C Receptor.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-QVENLELPVN

    , corresponding to amino acids 437-446 of Human 5HT2C Receptor.

  • Positive control
    • Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.

Properties

Applications

Our Abpromise guarantee covers the use of ab32887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
ELISA 1/64000.
IHC-Fr Use at an assay dependent concentration. Abreview 30686 suggests that this antibody is not suitable for this application

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Domain
    The PDZ domain-binding motif is involved in the interaction with MPDZ.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 Hydroxytryptamine 2C receptor antibody
    • 5-HT-1C antibody
    • 5-ht-1c receptor antibody
    • 5-HT-2C antibody
    • 5-HT1C antibody
    • 5-HT2C antibody
    • 5-HTR2C antibody
    • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
    • 5-hydroxytryptamine receptor 1C antibody
    • 5-hydroxytryptamine receptor 2C antibody
    • 5HT1C antibody
    • 5HT2C antibody
    • 5HT2C_HUMAN antibody
    • 5HTR2C antibody
    • 5Hydroxytryptamine 2C receptor antibody
    • Htr1c antibody
    • HTR2C antibody
    • serotonin 1c receptor antibody
    • serotonin 2c receptor antibody
    • Serotonin 5-HT-2C receptor antibody
    • Serotonin receptor 2C antibody
    see all

Anti-5HT2C Receptor antibody images

  • Anti-5HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-5HT2C Receptor antibody (ab32887)

ab32887 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Great news! The blocking peptide has been added as 5HT2C Receptor peptide ab168550.

http://www.abcam.com/5ht2c-receptor-peptide-ab168550.html

I hope this information helps. Please contact us wit...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
formalin
Antigen retrieval step
Enzymatic
Permeabilization
Yes - 1xPBT
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2012


I am very pleased to hear you would like to accept our offer and test ab32887 and ab44640 in IHC-FoFr and WB, respectively. These codes will give you: 1 free primary antibody each before the expiration date. To redeem this offer, please submit an ...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (hypothalamus PVN sections)
Specification
hypothalamus PVN sections
Fixative
ethanol 70%
Permeabilization
Yes - triton-x
Blocking step
2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Ms. Rebecca Ring

Verified customer

Submitted Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up