• Product nameAnti-5HT3A receptor antibody
    See all 5HT3A receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT3A receptor
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human 5HT3A receptor.

    (Peptide available as ab45313.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Brain, Testis, Heart and Skeletal Muscle.



Our Abpromise guarantee covers the use of ab45314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects bands of approximately 40 and 55 kDa (predicted molecular weight: 55 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • 5 HT 3 antibody
      • 5 HT3 receptor antibody
      • 5 HT3A antibody
      • 5 HT3A receptor antibody
      • 5 HT3RA antibody
      • 5 hydroxytryptamine (serotonin) receptor 3A antibody
      • 5-HT-3 antibody
      • 5-HT3-A antibody
      • 5-HT3A antibody
      • 5-HT3R antibody
      • 5-hydroxytryptamine receptor 3 antibody
      • 5-hydroxytryptamine receptor 3A antibody
      • 5HT receptor antibody
      • 5HT3 serotonin receptor antibody
      • 5HT3A_HUMAN antibody
      • 5HT3R antibody
      • HTR3 antibody
      • Htr3a antibody
      • Serotonin receptor 3A antibody
      • Serotonin-gated ion channel receptor 3a antibody
      • Serotonin-gated ion channel receptor antibody
      see all

    Anti-5HT3A receptor antibody images

    • All lanes : Anti-5HT3A receptor antibody (ab45314) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Human testis tissue lysate - total protein (ab30257)
      Lane 3 : Human heart tissue lysate - total protein (ab29431)
      Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

      Lysates/proteins at 10 µg per lane.

      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 40,55 kDa (why is the actual band size different from the predicted?)
    • IHC image of 5HT3A staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45314, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    References for Anti-5HT3A receptor antibody (ab45314)

    This product has been referenced in:
    • Ozcan CU  et al. Evaluation of the relation between interstitial cells of cajal (CD117) and serotonin receptor (5HT-3A) with postfundoplication dysphagia. Int J Surg 13:137-41 (2015). Read more (PubMed: 25498492) »

    See 1 Publication for this product

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