Anti-5HT4 Receptor antibody (ab60359)

Overview

  • Product nameAnti-5HT4 Receptor antibody
    See all 5HT4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT4 Receptor
  • Specificity5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide: LMAILGNLLV MVAVCWDRQ L RKIKTNYF IV SLAFADL LVS, corresponding to amino acids 31/70 of Human 5HT4 Receptor

  • Positive control
    • 293 cells, treated with insulin (0.01U/ml, 15mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab60359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • RelevanceThe 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression.
  • Cellular localizationCell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
  • Database links
  • Alternative names
    • 5 HT 4 antibody
    • 5 HT4 antibody
    • 5 HT4R antibody
    • 5 hydroxytryptamine 4 receptor antibody
    • 5 hydroxytryptamine receptor 4 antibody
    • 5 hydroxytryptamine serotonin receptor 4 antibody
    • 5 hydroxytryptamine4 receptor antibody
    • 5-HT-4 antibody
    • 5-ht4 antibody
    • 5-HT4R antibody
    • 5H4 antibody
    • 5HT 4 antibody
    • 5HT4 antibody
    • 5ht4 receptor antibody
    • 5HT4R antibody
    • Cardiac 5 HT4 receptor antibody
    • HTR 4 antibody
    • HTR4 antibody
    • serotonin 4 receptor antibody
    • Serotonin 5 HT4 receptor antibody
    • serotonin 5-ht-4 receptor antibody
    • serotonin 5-ht-4a antibody
    • Serotonin receptor 4 antibody
    see all

Anti-5HT4 Receptor antibody images

  • All lanes : Anti-5HT4 Receptor antibody (ab60359)

    Lane 1 : 293 cells treated with insulin (0.01U/ml, 15 mins)
    Lane 2 : 293 cells treated with insulin (0.01U/ml, 15 mins) with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
  • ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right).
  • All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-5HT4 Receptor antibody (ab60359)

ab60359 has not yet been referenced specifically in any publications.

Product Wall

I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human adrenal, brain, lung)
Specification human adrenal, brain, lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization No
Blocking step Ab94737 kit blocking agent as blocking agent for 10 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
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Submitted Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"