Overview

  • Product name
    Anti-5HT4 Receptor antibody
    See all 5HT4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT4 Receptor
  • Host species
    Rabbit
  • Specificity
    5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide: LMAILGNLLV MVAVCWDRQ L RKIKTNYF IV SLAFADL LVS, corresponding to amino acids 31/70 of Human 5HT4 Receptor

  • Positive control
    • 293 cells, treated with insulin (0.01U/ml, 15mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab60359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Relevance
    The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
  • Database links
  • Alternative names
    • 5 HT 4 antibody
    • 5 HT4 antibody
    • 5 HT4R antibody
    • 5 hydroxytryptamine (serotonin) receptor 4, G protein coupled antibody
    • 5 hydroxytryptamine 4 receptor antibody
    • 5 hydroxytryptamine receptor 4 antibody
    • 5 hydroxytryptamine serotonin receptor 4 antibody
    • 5 hydroxytryptamine4 receptor antibody
    • 5-HT-4 antibody
    • 5-ht4 antibody
    • 5-HT4R antibody
    • 5H4 antibody
    • 5HT 4 antibody
    • 5HT4 antibody
    • 5ht4 receptor antibody
    • 5HT4R antibody
    • Cardiac 5 HT4 receptor antibody
    • HTR 4 antibody
    • HTR4 antibody
    • serotonin 4 receptor antibody
    • Serotonin 5 HT4 receptor antibody
    • serotonin 5-ht-4 receptor antibody
    • serotonin 5-ht-4a antibody
    • Serotonin receptor 4 antibody
    see all

Images

  • All lanes : Anti-5HT4 Receptor antibody (ab60359)

    Lane 1 : 293 cells treated with insulin (0.01U/ml, 15 mins)
    Lane 2 : 293 cells treated with insulin (0.01U/ml, 15 mins) with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 44 kDa

  • ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right).
  • All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 42 kDa (why is the actual band size different from the predicted?)


    Exposure time: 5 minutes

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human adrenal, brain, lung)
Specification
human adrenal, brain, lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
No
Blocking step
Ab94737 kit blocking agent as blocking agent for 10 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
Username

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Verified customer

Submitted Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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