Overview

  • Product name
    Anti-5HT4 Receptor antibody
    See all 5HT4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT4 Receptor
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human 5HT4 Receptor.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Caco 2 Whole Cell Lysate; SW480 Whole Cell Lysate; HeLa Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

  • Relevance
    The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
  • Database links
  • Alternative names
    • 5 HT 4 antibody
    • 5 HT4 antibody
    • 5 HT4R antibody
    • 5 hydroxytryptamine (serotonin) receptor 4, G protein coupled antibody
    • 5 hydroxytryptamine 4 receptor antibody
    • 5 hydroxytryptamine receptor 4 antibody
    • 5 hydroxytryptamine serotonin receptor 4 antibody
    • 5 hydroxytryptamine4 receptor antibody
    • 5-HT-4 antibody
    • 5-ht4 antibody
    • 5-HT4R antibody
    • 5H4 antibody
    • 5HT 4 antibody
    • 5HT4 antibody
    • 5ht4 receptor antibody
    • 5HT4R antibody
    • Cardiac 5 HT4 receptor antibody
    • HTR 4 antibody
    • HTR4 antibody
    • serotonin 4 receptor antibody
    • Serotonin 5 HT4 receptor antibody
    • serotonin 5-ht-4 receptor antibody
    • serotonin 5-ht-4a antibody
    • Serotonin receptor 4 antibody
    see all

Images

  • All lanes : Anti-5HT4 Receptor antibody (ab87337) at 2 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab87337 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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