Overview

  • Product nameAnti-5T4 antibody
    See all 5T4 primary antibodies
  • Description
    Mouse monoclonal to 5T4
  • Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 219-329 of Human 5T4, with a 26 KDa tag (NP_006661).

  • Positive control
    • HeLa and PC12 cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24736661ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 24736661

Target

  • Tissue specificityExpressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
  • Sequence similaritiesContains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Form5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).
  • Alternative names
    • 5T4 antibody
    • 5T4 oncofetal antigen antibody
    • 5T4 oncofetal trophoblast glycoprotein antibody
    • 5T4 oncotrophoblast glycoprotein antibody
    • 5T4AG antibody
    • AW495680 antibody
    • M6P1 antibody
    • TPBG antibody
    • TPBG_HUMAN antibody
    • Trophoblast glycoprotein antibody
    see all

Anti-5T4 antibody images

  • Anti-5T4 antibody (ab88091) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Anti-5T4 antibody (ab88091) at 5 µg/ml + PC12 cell lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Detection limit for ab88091 is approximately 1ng/ml as a capture antibody.

References for Anti-5T4 antibody (ab88091)

This product has been referenced in:
  • Patterson KG  et al. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen. PLoS One 9:e95200 (2014). IF, Flow Cyt ; Human . Read more (PubMed: 24736661) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"