Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582)

Overview

  • Product nameAnti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide freeSee all 67kDa Laminin Receptor primary antibodies ...
  • Description
    Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor - BSA and Azide free
  • Tested applicationsIHC-P, Flow Cyt, Inhibition Assay, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Small cell lung carcinoma N592 live cells.

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.
Inhibition Assay Use at an assay dependent dilution. Partially inhibits the binding of soluble laminin.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
  • Sequence similaritiesBelongs to the ribosomal protein S2P family.
  • Post-translational
    modifications
    Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
    Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
  • Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
  • Target information above from: UniProt accession P08865 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 34/67 kDa laminin receptor antibody
    • 37 kDa laminin receptor precursor antibody
    • 37/67 kDa laminin receptor antibody
    • 37LRP antibody
    • 40S ribosomal protein SA antibody
    • 67 kDa laminin receptor antibody
    • 67LR antibody
    • Colon carcinoma laminin binding protein antibody
    • Colon carcinoma laminin-binding protein antibody
    • LAMBR antibody
    • Laminin Receptor 1 antibody
    • Laminin-binding protein precursor p40 antibody
    • LAMR 1 antibody
    • LamR antibody
    • LAMR1 antibody
    • LBP antibody
    • LBP/p40 antibody
    • LRP antibody
    • LRP/LR antibody
    • Multidrug resistance associated protein MGr1 Ag antibody
    • Multidrug resistance associated protein MGr1Ag antibody
    • Multidrug resistance-associated protein MGr1-Ag antibody
    • NEM/1CHD4 antibody
    • p40 antibody
    • Ribosomal Protein SA antibody
    • RPSA antibody
    • RSSA_HUMAN antibody
    • SA antibody
    see all

Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free images

  • Formalin-fixed, paraffin-embedded human breast carcinoma stained with ab80582 at 8µg/ml using a peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.
  • Overlay histogram showing MCF7 cells stained with ab80582 (red line). The cells were fixed with 4% paraformaldehyde (10 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80582, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582)

This product has been referenced in:
  • Lee JH  et al. Vitamin A enhances antitumor effect of a green tea polyphenol on melanoma by upregulating the polyphenol sensing molecule 67-kDa laminin receptor. PLoS One 5:e11051 (2010). Inhibition Assay ; Mouse . Read more (PubMed: 20548792) »
  • Martignone S  et al. Characterization of two monoclonal antibodies directed against the 67 kDa high affinity laminin receptor and application for the study of breast carcinoma progression. Clin Exp Metastasis 10:379-86 (1992). Inhibition Assay . Read more (PubMed: 1333381) »

See all 2 Publications for this product

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I am very sorry for the delayed response.
This antibody is sourced and unfortunately we have not testedit in inhibition assay in-house. According to this published paper, ab3099 (clone M...

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