![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582) image](#67kDa-Laminin-Receptor-Primary-antibodies-ab80582-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free
See all 67kDa Laminin Receptor products (10) ...
Description
Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor - BSA and Azide free
Tested applications
IHC-P, Flow Cyt, Inhibition Assay, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Small cell lung carcinoma N592 live cells.
Positive control
Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
MLuC5
Isotype
IgM
Research areas
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Neuroscience >> Neurology process >> Neurodegenerative disease >> Prions
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582) image](/ps/datasheet/images/80/ab80582/67kDa-Laminin-Receptor-Primary-antibodies-ab80582-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 - 8 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt: Use at an assay dependent dilution.
Inhib: Use at an assay dependent dilution. (Partially inhibits the binding of soluble laminin. )
ICC/IF: Use at an assay dependent dilution.
Target
Function
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
Sequence similarities
Belongs to the ribosomal protein S2P family.
Post-translational
modifications
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
Cellular localization
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
Target information above from: UniProt accessionP08865
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
see all
Database links
- Entrez Gene: 3921 Human
- Omim: 150370 Human
- SwissProt: P08865 Human
- SwissProt: Q6NXQ8 Human
- Unigene: 449909 Human
Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582)
Formalin-fixed, paraffin-embedded human breast carcinoma stained with ab80582 at 8µg/ml using a peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.
References for Anti-67kDa Laminin Receptor antibody [MLuC5] - BSA and Azide free (ab80582)
This product has been referenced in:
- Lee JHet al. Vitamin A enhances antitumor effect of a green tea polyphenol on melanoma by upregulating the polyphenol sensing molecule 67-kDa laminin receptor. PLoS One 5:e11051 (2010). Inhibition Assay; Mouse.Read more (PubMed: 20548792) »
- Martignone Set al. Characterization of two monoclonal antibodies directed against the 67 kDa high affinity laminin receptor and application for the study of breast carcinoma progression. Clin Exp Metastasis 10:379-86 (1992). Inhibition Assay.Read more (PubMed: 1333381) »
See all 2 publications for this product
Publishing research using ab80582? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"