Anti-67kDa Laminin Receptor antibody (ab99484)

Overview

  • Product nameAnti-67kDa Laminin Receptor antibodySee all 67kDa Laminin Receptor primary antibodies ...
  • Description
    Rabbit polyclonal to 67kDa Laminin Receptor
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Zebra finch
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human 67kDa Laminin Receptor.

  • Positive control
    • This antibody gave a positive signal in Hu brain tissue lysate as well as the following whole cell lysates: Caco2; HCT116; LOVO; A549; HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab99484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).

Target

  • FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
  • Sequence similaritiesBelongs to the ribosomal protein S2P family.
  • Post-translational
    modifications
    Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
    Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
  • Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 34/67 kDa laminin receptor antibody
    • 37 kDa laminin receptor precursor antibody
    • 37/67 kDa laminin receptor antibody
    • 37LRP antibody
    • 40S ribosomal protein SA antibody
    • 67 kDa laminin receptor antibody
    • 67LR antibody
    • Colon carcinoma laminin binding protein antibody
    • Colon carcinoma laminin-binding protein antibody
    • LAMBR antibody
    • Laminin Receptor 1 antibody
    • Laminin-binding protein precursor p40 antibody
    • LAMR 1 antibody
    • LamR antibody
    • LAMR1 antibody
    • LBP antibody
    • LBP/p40 antibody
    • LRP antibody
    • LRP/LR antibody
    • Multidrug resistance associated protein MGr1 Ag antibody
    • Multidrug resistance associated protein MGr1Ag antibody
    • Multidrug resistance-associated protein MGr1-Ag antibody
    • NEM/1CHD4 antibody
    • p40 antibody
    • Ribosomal Protein SA antibody
    • RPSA antibody
    • RSSA_HUMAN antibody
    • SA antibody
    see all

Anti-67kDa Laminin Receptor antibody images

  • All lanes : Anti-67kDa Laminin Receptor antibody (ab99484) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab99484 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99484 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, HEk293, and MCF-7 cells at 1ug/ml, and in formaldehyde fixed (4%, 10min) HeLa, HEk293, HepG2, and MCF-7 cells at 1ug/ml.

References for Anti-67kDa Laminin Receptor antibody (ab99484)

ab99484 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99484.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"