Anti-67kDa Laminin Receptor antibody (ab99484)

Overview

• Product nameAnti-67kDa Laminin Receptor antibodySee all 67kDa Laminin Receptor primary antibodies ...
• Description
  Rabbit polyclonal to 67kDa Laminin Receptor
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Zebra finch
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human 67kDa Laminin Receptor.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in Hu brain tissue lysate as well as the following whole cell lysates: Caco2; HCT116; LOVO; A549; HeLa.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Prions

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Cell Adhesion Proteins
  • ECM Proteins

Applications

Target

• FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
• Sequence similaritiesBelongs to the ribosomal protein S2P family.
• Post-translational
  modificationsAcylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
  Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
• Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.

Target information above from: UniProt accession P08865 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281898 Cow
  • Entrez Gene: 3921 Human
  • Entrez Gene: 100505031 Mouse
  • Entrez Gene: 16785 Mouse
  • Entrez Gene: 641351 Pig
  • Entrez Gene: 29236 Rat
  • Omim: 150370 Human
  • SwissProt: P26452 Cow

  • SwissProt: P08865 Human
  • SwissProt: P14206 Mouse
  • SwissProt: Q4GWZ2 Pig
  • SwissProt: P38983 Rat
  • Unigene: 449909 Human
  • Unigene: 311972 Mouse
  • Unigene: 391708 Mouse
  • Unigene: 4071 Mouse
  • Unigene: 121972 Rat
  • Unigene: 161973 Rat
  • Unigene: 999 Rat

  see all

• Alternative names
  34/67 kDa laminin receptor antibody37 kDa laminin receptor precursor antibody37/67 kDa laminin receptor antibody

  37LRP antibody40S ribosomal protein SA antibody67 kDa laminin receptor antibody67LR antibodyColon carcinoma laminin binding protein antibodyColon carcinoma laminin-binding protein antibodyLAMBR antibodyLaminin Receptor 1 antibodyLaminin-binding protein precursor p40 antibodyLAMR 1 antibodyLamR antibodyLAMR1 antibodyLBP antibodyLBP/p40 antibodyLRP antibodyLRP/LR antibodyMultidrug resistance associated protein MGr1 Ag antibodyMultidrug resistance associated protein MGr1Ag antibodyMultidrug resistance-associated protein MGr1-Ag antibodyNEM/1CHD4 antibodyp40 antibodyRibosomal Protein SA antibodyRPSA antibodyRSSA_HUMAN antibodySA antibody

  see all

Anti-67kDa Laminin Receptor antibody images

• 

  Western blot - 67kDa Laminin Receptor antibody (ab99484)
  All lanes : Anti-67kDa Laminin Receptor antibody (ab99484) at 1 µg/ml
  
  Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
  Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
  Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
  Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
  Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 33 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds
• 

  Immunocytochemistry/ Immunofluorescence - 67kDa Laminin Receptor antibody (ab99484)

  ICC/IF image of ab99484 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99484 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, HEk293, and MCF-7 cells at 1ug/ml, and in formaldehyde fixed (4%, 10min) HeLa, HEk293, HepG2, and MCF-7 cells at 1ug/ml.