|
|
|
|
- 67kD Laminin Receptor antibody (ab2508)
(enlarge)
|
|
- 67kD Laminin Receptor antibody (ab2508)
(enlarge)
|
|
Immunohistochemistry - Free Floating - 67kDa Laminin Receptor antibody (ab2508)
(enlarge)
|
|
|
|
|
Product Name
|
67kDa Laminin Receptor antibody
|
| |
See all 67kDa Laminin Receptor antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to 67kDa Laminin Receptor
|
|
Immunogen
|
Synthetic peptide derived from within residues 1 - 100 of Human 67kD Laminin Receptor.
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
This antibody was raised against the human 67LR N-terminal sequence SGALDVLQ which represents amino acids 2-9 precursor (37LRP or 'P40') (Castronovo et al.). This antibody recognises both denatured and native 67LR. Higher (110 kDa) and lower (50 kDa) bands are also detected in immunoprecipitates. Such bands have been noted before (Clement et al., Landowski et al.). The N-terminal ab2508 epitope appears to be intracellular, as non-permeablised cells do not stain with this antibody (Castronovo et al.).
|
|
Tested applications
(see key)
|
ICC/IF, IHC (PFA fixed), IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: 1/1000.
Immunohistochemistry (PFA fixed): 1/300 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cell Membrane and Cytoplasmic
|
|
Research areas
|
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Neuroscience >> Neurology process >> Neurodegenerative disease >> Prions
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
|
|
Relevance
|
Laminin, one of the major glycoproteins of basement membrane, displays multiple biological activities which are mediated through its interaction with specific cell membrane receptors. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein.
Overexpression of Laminin Receptor is reportedly an independent prognostic factor in breast cancer and correlates with the dissemination of the tumor cells in bone marrow. Laminin Receptors appears to play an important role in tumor invasion and metastasis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
We would advise researchers use Tris (2-carboxyethyl) phosphine hydrochloride ("TCEP", available from Sigma product number 93284) as a reducing agent at a final concentration of 5mM in the treatment buffer.
We believe this should produce much cleaner results than using DTT or mercaptoethanol with this antibody. Please see ab711 for further explanation.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 67kDa Laminin Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for 67kDa Laminin Receptor antibody images, references, products related to ab2508 and other tools.
|
| |
67kDa Laminin Receptor antibody images:
|
 - 67kD Laminin Receptor antibody (ab2508)
Crude cell lysate (from T-47D human breast cancer cells) was separated on 10-20% Tricine gel in denaturing conditions, then blotted and detected with 1/100 dilution of ab2508 followed by 1/5000 anti-rabbit HRP. John Nelson, Queen's University of Belfast
|
| |
- 67kD Laminin Receptor antibody (ab2508)
Crude cell lysate (from T-47D human breast cancer cells) was immunoprecipitated using ab2508 linked to anti-rabbit conjugated to magnetic beads.
ab2508-precipitated proteins were separated on 10-20% Tricine gel in denaturing conditions and then blotted. The blot was treated with ab711 (1/1000) then detected using 1/5000 anti-rabbit HRP. John Nelson, Queen's University of Belfast
|
| |
Immunohistochemistry - Free Floating - 67kDa Laminin Receptor antibody (ab2508)
Composite image showing immunofuorescent staining for Laminin receptor in the rat cortex and hippocampus. Images acquired with the objective X10. Recommended dilution of antibody: 1:300-1:1000 with overnight incubation at room temperature. Free floating IHC performed with TSA amplification. Tissue was 4% perfusion PFA-fixed brain. Sophie Pezet, King`s college London, United Kingdom
|
| |
Immunofluorescence - 67kDa Laminin Receptor antibody (ab2508)
Immunofluorescent staining for laminin receptor in the hippocampus. The staining is observed in the cytoplasm of some astrocytes and neurons. Images acquired with X20 objective. Recommended dilution of antibody= 1:300-1:1000 with overnight incubation at room temperature. Free floating IHC performed with TSA amplification. Tissue was 4% perfusion PFA-fixed brain. Sophie Pezet, King`s college London, United Kingdom
|
| |
Immunocytochemistry/ Immunofluorescence - 67kDa Laminin Receptor antibody (ab2508)
ICC/IF image of ab2508 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab2508, 1:1000 dilution) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.
|
| |
Immunocytochemistry/ Immunofluorescence - 67kDa Laminin Receptor antibody (ab2508)
ICC/IF image of ab2508 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2508, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
References for 67kDa Laminin Receptor antibody (ab2508)
|
|
This product has been used in:
Nagymanyoki Z et al. Overexpression of laminin receptor 1 on decidual cells in partial and complete mole. Gynecol Oncol 108:121-5 (2008). PubMed: 17928039
If you publish research using ab2508 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to 67kDa Laminin Receptor
|
| |
67kDa Laminin Receptor antibody - more information
|
Customer reviews (feedback) regarding 67kDa Laminin Receptor antibody |
Customer FAQs regarding 67kDa Laminin Receptor antibody |
Protocols for 67kDa Laminin Receptor antibody |
Price and availability of products related to 67kDa Laminin Receptor antibody |
| |
67kDa Laminin Receptor antibody - related products:
|
|
|
| |
67kDa Laminin Receptor antibody - other tools:
|
Contact Abcam with a Technical Enquiry about 67kDa Laminin Receptor antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
