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Immunocytochemistry/ Immunofluorescence-67kDa Laminin Receptor antibody [MLuC5](ab3099)
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Product Name
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67kDa Laminin Receptor antibody [MLuC5]
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See all 67kDa Laminin Receptor antibodies (9)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor
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Immunogen
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Small cell lung carcinoma N592 live cells.
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-P, Inhib
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 4 - 8 µg/ml for 30mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 mins followed by cooling for 20mins at RT).
Inhib: Use at an assay dependent dilution. This antibody partially inhibits the binding of soluble Laminin (Martignone S, et. al).
Is unsuitable for IHC-FoFr.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Neuroscience >> Neurology process >> Neurodegenerative disease >> Prions
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
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Relevance
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Laminin, one of the major glycoproteins of basement membrane, displays multiple biological activities which are mediated through its interaction with specific cell membrane receptors. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein.
Overexpression of Laminin Receptor is reportedly an independent prognostic factor in breast cancer and correlates with the dissemination of the tumor cells in bone marrow. Laminin Receptors appears to play an important role in tumor invasion and metastasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MLuC5
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Isotype
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IgM
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites by ammonium sulfate precipitation.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Molecular weight of Laminin Receptor = ~ 67 kDa.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 67kDa Laminin Receptor antibody [MLuC5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 67kDa Laminin Receptor antibody [MLuC5] images, references, products related to ab3099 and other tools.
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67kDa Laminin Receptor antibody [MLuC5] images:
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 Immunocytochemistry/ Immunofluorescence-67kDa Laminin Receptor antibody [MLuC5](ab3099)
ICC/IF image of ab3099 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for 67kDa Laminin Receptor antibody [MLuC5] (ab3099)
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This product has been used in: (two most recent references)
Orihuela CJ et al. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. J Clin Invest : (2009). IHC-Fr; Mouse. PubMed: 19436113
Okaji Y et al. Effects of down-regulating the Id genes in human colorectal cancer cells on early steps of haematogenous metastasis. Eur J Cancer 42:668-73 (2006). PubMed: 16427272
See all 3 publication references for this product.
If you publish research using ab3099 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 67kDa Laminin Receptor
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67kDa Laminin Receptor antibody [MLuC5] - more information
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67kDa Laminin Receptor antibody [MLuC5] - related products:
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67kDa Laminin Receptor antibody [MLuC5] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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