Anti-67kDa Laminin Receptor antibody [MLuC5] (ab80582)


  • Product nameAnti-67kDa Laminin Receptor antibody [MLuC5]
    See all 67kDa Laminin Receptor primary antibodies
  • Description
    Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor
  • Tested applicationsIHC-P, Flow Cyt, Inhibition Assay, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Small cell lung carcinoma N592 live cells.

  • Positive control
    • Breast carcinoma.



Our Abpromise guarantee covers the use of ab80582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. Partially inhibits the binding of soluble laminin.
ICC/IF Use at an assay dependent concentration.


  • FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
  • Sequence similaritiesBelongs to the ribosomal protein S2P family.
  • Post-translational
    Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
    Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
  • Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 34/67 kDa laminin receptor antibody
    • 37 kDa laminin receptor precursor antibody
    • 37/67 kDa laminin receptor antibody
    • 37LRP antibody
    • 40S ribosomal protein SA antibody
    • 67 kDa laminin receptor antibody
    • 67LR antibody
    • Colon carcinoma laminin binding protein antibody
    • Colon carcinoma laminin-binding protein antibody
    • LAMBR antibody
    • Laminin Receptor 1 antibody
    • Laminin-binding protein precursor p40 antibody
    • LAMR 1 antibody
    • LamR antibody
    • LAMR1 antibody
    • LBP antibody
    • LBP/p40 antibody
    • LRP antibody
    • LRP/LR antibody
    • Multidrug resistance associated protein MGr1 Ag antibody
    • Multidrug resistance associated protein MGr1Ag antibody
    • Multidrug resistance-associated protein MGr1-Ag antibody
    • NEM/1CHD4 antibody
    • p40 antibody
    • Ribosomal Protein SA antibody
    • RPSA antibody
    • RSSA_HUMAN antibody
    • SA antibody
    see all

Anti-67kDa Laminin Receptor antibody [MLuC5] images

  • Formalin-fixed, paraffin-embedded human breast carcinoma stained with ab80582 at 8µg/ml using a peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.
  • Overlay histogram showing MCF7 cells stained with ab80582 (red line). The cells were fixed with 4% paraformaldehyde (10 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80582, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-67kDa Laminin Receptor antibody [MLuC5] (ab80582)

This product has been referenced in:
  • Lee JH  et al. Vitamin A enhances antitumor effect of a green tea polyphenol on melanoma by upregulating the polyphenol sensing molecule 67-kDa laminin receptor. PLoS One 5:e11051 (2010). Inhibition Assay ; Mouse . Read more (PubMed: 20548792) »
  • Martignone S  et al. Characterization of two monoclonal antibodies directed against the 67 kDa high affinity laminin receptor and application for the study of breast carcinoma progression. Clin Exp Metastasis 10:379-86 (1992). Inhibition Assay . Read more (PubMed: 1333381) »

See all 2 Publications for this product

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This antibody is sourced and unfortunately we have not testedit in inhibition assay in-house. According to this published paper, ab3099 (clone M...

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