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RabMAbs (1815-1)

Anti-68kDa Neurofilament antibody [EP675Y] (ab52989)

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Overview

  • Product nameAnti-68kDa Neurofilament antibody [EP675Y]See all 68kDa Neurofilament primary antibodies ...
  • Description
    Rabbit monoclonal [EP675Y] to 68kDa Neurofilament
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen

  • Positive controlSH-SY5Y cytoplasmic cell lysate
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab52989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: 1/70.
    IP: 1/50.
    WB: 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).

    Is unsuitable for ICC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    • Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
      Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
    • Post-translational
      modifications      O-glycosylated.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

      • Target information above from: UniProt accession P07196 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        68 kDa neurofilament protein antibody68kDa neurofilament protein antibodyCMT 1F antibody
        CMT 2E antibodyCMT1F antibodyCMT2E antibodyFLJ53642 antibodyLight molecular weight neurofilament protein antibodyNEFL antibodyNeurofilament light antibodyNeurofilament light polypeptide 68kDa antibodyNeurofilament light polypeptide antibodyNeurofilament protein, light chain antibodyNeurofilament subunit NF L antibodyNeurofilament triplet L protein antibodyNF 68 antibodyNF L antibodyNF-L antibodyNF68 antibodyNFL antibodyNFL_HUMAN antibody
      see all

    Anti-68kDa Neurofilament antibody [EP675Y] images

    • Western blot - 68kDa Neurofilament antibody [EP675Y] - Carboxyterminal end (ab52989)
      Western blot - 68kDa Neurofilament antibody [EP675Y] - Carboxyterminal end (ab52989)
      Anti-68kDa Neurofilament antibody [EP675Y] (ab52989) at 1/50000 dilution + SH-SY5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    References for Anti-68kDa Neurofilament antibody [EP675Y] (ab52989)

    This product has been referenced in:
    • Zhao X  et al. Neuronal PPARgamma deficiency increases susceptibility to brain damage after cerebral ischemia. J Neurosci 29:6186-95 (2009). WB ; Mouse . Read more (PubMed: 19439596) »

    See 1 Publication for this product

    Publishing research using ab52989 ? Please let us know so that we can cite the reference in this datasheet

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