Anti-68kDa Neurofilament/NF-L antibody (ab113854)
Key features and details
- Rabbit polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Rabbit polyclonal to 68kDa Neurofilament/NF-L -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Chicken, Cow, Dog, Pig, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Rat Cerebellum; Human Spinal Cord; Mouse Spinal Cord.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Involvement in disease
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
Post-translational
modificationsO-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 419528 Chicken
- Entrez Gene: 281348 Cow
- Entrez Gene: 4747 Human
- Entrez Gene: 18039 Mouse
- Entrez Gene: 100173482 Orangutan
- Entrez Gene: 100521224 Pig
- Entrez Gene: 83613 Rat
- Omim: 162280 Human
see all -
Alternative names
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
Images
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All lanes : Anti-68kDa Neurofilament/NF-L antibody (ab113854) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Cerebellum Rat Tissue Lysate
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes68kDa Neurofilament/NF-L contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab113854 has not yet been referenced specifically in any publications.