Anti-68kDa Neurofilament/NF-L antibody (ab24520)
Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Cow
- Isotype: IgY
Overview
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Product name
Anti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Chicken polyclonal to 68kDa Neurofilament/NF-L -
Host species
Chicken -
Tested applications
Suitable for: IHC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow -
Immunogen
Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L.
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General notes
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purification notes
The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation. -
Primary antibody notes
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC |
1/2000.
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WB |
1/20000. Predicted molecular weight: 68 kDa.
Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries. |
Notes |
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IHC
1/2000. |
WB
1/20000. Predicted molecular weight: 68 kDa. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries. |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Involvement in disease
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
Post-translational
modificationsO-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 281348 Cow
- Entrez Gene: 18039 Mouse
- Entrez Gene: 83613 Rat
- SwissProt: P02548 Cow
- SwissProt: P08551 Mouse
- SwissProt: P19527 Rat
- Unigene: 1956 Mouse
- Unigene: 18568 Rat
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Alternative names
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
Images
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All lanes : Anti-68kDa Neurofilament/NF-L antibody (ab24520) at 1/20000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 68 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed (transcardial perfusion) of free floating section of rat cerecellum tissue stained for 68kDa Neurofilament/NF-L (green) using ab24520 at 1/200 dilution. FOX3/NeuN is stained with an anti-FOX3/NeuN antibody (red).
Datasheets and documents
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Datasheet download
References (0)
ab24520 has not yet been referenced specifically in any publications.