Anti-68kDa Neurofilament/NF-L antibody (ab72997)
Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgY
Overview
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Product name
Anti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Chicken polyclonal to 68kDa Neurofilament/NF-L -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L. Bovine spinal cord intermediate filaments.
Database link: P02548 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Involvement in disease
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
Post-translational
modificationsO-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 83613 Rat
- SwissProt: P19527 Rat
- Unigene: 18568 Rat
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Alternative names
- 68 kDa neurofilament protein antibody
- 68kDa Neurofilament antibody
- 68kDa neurofilament protein antibody
see all
Images
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Anti-68kDa Neurofilament/NF-L antibody (ab72997) at 1/10000 dilution + Rat cerebral cortex lysate
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (11)
ab72997 has been referenced in 11 publications.
- Gras S et al. Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system. Aging (Albany NY) 13:18051-18093 (2021). PubMed: 34319911
- Damián JP et al. Central Alteration in Peripheral Neuropathy of Trembler-J Mice: Hippocampal pmp22 Expression and Behavioral Profile in Anxiety Tests. Biomolecules 11:N/A (2021). PubMed: 33921657
- Kim B et al. Ontogeny of inter-alpha inhibitor protein (IAIP) expression in human brain. J Neurosci Res 98:869-887 (2020). PubMed: 31797408
- Blasco A et al. Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice. J Cachexia Sarcopenia Muscle 11:1628-1660 (2020). PubMed: 32691534
- Karbassi E et al. Direct visualization of cardiac transcription factories reveals regulatory principles of nuclear architecture during pathological remodeling. J Mol Cell Cardiol 128:198-211 (2019). PubMed: 30742811