Anti-68kDa Neurofilament antibody [2F11] (ab8970)

Overview

  • Product nameAnti-68kDa Neurofilament antibody [2F11]
    See all 68kDa Neurofilament primary antibodies
  • Description
    Mouse monoclonal [2F11] to 68kDa Neurofilament
  • Specificityab8970 reacts with the phosphorylated isoform of the 68kDa neurofilament protein.

    Some labs have reported an additional 200 kDa band under certain conditions. We cannot confirm if this is in fact a multimer of the 68 kDa protein.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human, Opossum
  • Immunogen

    Human neurofilament preparation.

  • General notesIn originator's published literature (e.g. PMID 6206727), this clone 2F11 has been shown specific to 68kDa protein however our testing on two different occasions show a 200 kDa protein band in western blot, under reduced and denaturing conditions. The 200 kDa could be either the glycosylated form of the 68kDa or 200kD a protein however we haven't done any further test to confirm it. We will welcome any feedback from researchers have who used the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab8970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/100 - 1/1000.

Target

  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa neurofilament protein antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

References for Anti-68kDa Neurofilament antibody [2F11] (ab8970)

This product has been referenced in:
  • Breckenridge LJ  et al. Developmentally regulated markers in the postnatal cervical spinal cord of the opossum Monodelphis domestica. Brain Res Dev Brain Res 103:47-57 (1997). Read more (PubMed: 9370059) »
  • van Muijen GN  et al. Cytokeratin and neurofilament in lung carcinomas. Am J Pathol 116:363-9 (1984). Read more (PubMed: 6206727) »

See all 2 Publications for this product

Product Wall

Thanks you for your email. Apologies for delay.

http://onlinelibrary.wiley.com/doi/10.1002/bit.260420917/pdf

This paper talks about how non producing cells can overtake a population and how it can potentially be resolved. It also men...

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Thank you for contacting us.

ab74592; This is to inform you that we have retested the product from the latest two bulk lots that we have, and using IMR5 cell lysate, we have detected 2 bands – 200 and around 70 kDa.

ab910: This ...

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Positive control for ab8970: cell cultures of neural tissues. These are references for the controls: 1. Kluck, P., van Muijen, G. N., van der Kamp, A. W., Tibboel, D., van Hoorn, W. A., Warnaar, S. O., and Molenaar, J. C. (1984). Hirschsprung's di...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"