Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035)

Overview

  • Product nameAnti-68kDa Neurofilament antibody - Neuronal Marker
    See all 68kDa Neurofilament primary antibodies
  • Description
    Rabbit polyclonal to 68kDa Neurofilament - Neuronal Marker
  • SpecificitySpecifically recognizes the light neurofilament subunit (~68 kDa).
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Pig
    Predicted to work with: Bird, all Mammals
  • Immunogen

    Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.

Applications

Our Abpromise guarantee covers the use of ab9035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-FoFr
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC/IF: 1/500.
    IHC-F: 1/1000.
    IHC-P: 1/1000.
    IHC-Fr: 1/1000.
    WB: 1/5000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    • Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
      Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
    • Post-translational
      modifications
      O-glycosylated.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt
    • Database links
    • Alternative names
      • 68 kDa neurofilament protein antibody
      • 68kDa neurofilament protein antibody
      • CMT1F antibody
      • CMT2E antibody
      • FLJ53642 antibody
      • Light molecular weight neurofilament protein antibody
      • NEFL antibody
      • Neurofilament light antibody
      • Neurofilament light polypeptide 68kDa antibody
      • Neurofilament light polypeptide antibody
      • Neurofilament protein, light chain antibody
      • Neurofilament subunit NF L antibody
      • Neurofilament triplet L protein antibody
      • NF-L antibody
      • NF68 antibody
      • NFL antibody
      • NFL_HUMAN antibody
      see all

    Anti-68kDa Neurofilament antibody - Neuronal Marker images

    • ab9035 staining anti 68kDa Neurofilament in mixed neuron/glia cultures from newborn rat brain by ICC/IF (Immunocytochemistry/immunofluorescence). Samples were incubated with primary antibody (1/500) (red) and co-stained with ab4573 for anti Peripherin (green).

    • Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035) at 1/20000 dilution + whole rat cerebellum homogenateA prominent band at ~68kDa corresponds to rodent NF-L. Human, cow and bovine NF-L run a little slower, at about 70kDa.
    • ab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine.

      See Abreview

    References for Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035)

    This product has been referenced in:
    • Clarke WT  et al. Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons. J Cell Sci : (2010). WB ; Mouse . Read more (PubMed: 20587592) »
    • Clements KM  et al. Cellular and histopathological changes in the infrapatellar fat pad in the monoiodoacetate model of osteoarthritis pain. Osteoarthritis Cartilage 17:805-12 (2009). Read more (PubMed: 19114312) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Sample Chicken Tissue sections (Hindbrain section)
    Specification Hindbrain section
    Permeabilization Yes - PBS + 0.1% Tween20
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 21 2014

    Thank you for your reply.

    Trying the antibody in WB is a good alternative to find out whether there might have been a problem with it. For WB, we recommend a 1/5000 dilution, and it should recognise a band at ˜68KDa under denaturing and reduc...

    Read More

    Thank you for your reply.

    I can confirm that without controls it is not possible to interpret the results. Also this antibody is not experimentallytested for mouse samples. Although it is very likely that ab9035 works with mouse samples, in t...

    Read More

    Thank you for your inquiry.

    Unfortunately, I am unable to provide a straight forward answer to your question. Since you are treating cells for neuronal differentiation and I assume that the untreated cells are supposed to be negative for the ...

    Read More

    Thanks for your inquiry. I checked with the lab and this is what they had to say: "The sample was serum, but in that particular batch there was some hemolysis resulting in release of some hemoglobin from red blood cells. We try to avoid s...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Hippocampal Organotypic Slice Cultures)
    Specification Hippocampal Organotypic Slice Cultures
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1.5%
    Username

    Dr. Luis Craveiro

    Verified customer

    Submitted Jun 30 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"