Anti-6X His tag® antibody [33D10.D2] (Biotin) (ab106261)

Overview

• Product nameAnti-6X His tag® antibody [33D10.D2] (Biotin)See all 6X His tag® primary antibodies ...
• Description
  Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin)
• ConjugationBiotin
• Conjugation notes10-20 BAC molecules per Mouse Monoclonal Antibody
• Specificityab106261 detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino or carboxy-termini of targeted proteins in transfected or transformed cells.
• Tested applicationsWB, ELISA more details
• Immunogen

  6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 1% BSA, 0.27% Potassium phosphate, 0.88% Sodium chloride
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number33D10.D2
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Epitope Tags
  • His Tag

Applications

Target

• RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
• Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.

• Alternative names
  6 His epitope tag antibodyHexa His tag antibodyHHHHHH epitope tag antibody

  HHHHHH tag antibodyHis tag antibody

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