Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Specificity
ab81652 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag®. Polyhistidine (HHHHHH) tagged polypeptide.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000.
|
Notes |
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WB
1/5000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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All lanes : Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652) at 1/10000 dilution
Lane 1 : 60 kDa 6X His-tagged protein at 0.3 µg
Lane 2 : 60 kDa 6X His-tagged protein at 0.1 µg
Lane 3 : 60 kDa 6X His-tagged protein at 0.03 µg
Lane 4 : 60 kDa 6X His-tagged protein at 0.01 µg
Lane 5 : 60 kDa 6X His-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab81652 has not yet been referenced specifically in any publications.