Products:Tags & Cell Markers >> Epitope Tags >> His Tag
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ab46666 |
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ab9108 has been referenced in 19 publications.
Publishing research using ab9108? Please let us know so that we can cite the reference in this datasheet
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A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate
All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution
Lane 1 : 10ug non-transformed lysate
Lane 2 : 10ug non-transformed lysate + 1mM IPTG
Lane 3 : 10ug E.coli Lysate harbouring His-tag-RelA NTD, non-induced
Lane 4 : 10ug Induction of His-tag RelA NTD 1 mM IPTG
Lane 5 : 10ug Induction of His-tag RelA NTD 1 mM IPTG
Secondary
HRP conjugated goat anti-rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 12 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an Abreview.
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