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Anti-6X His tag® antibody - ChIP Grade (ab9108)

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Overview

Product name

Anti-6X His tag® antibody - ChIP Grade
See all 6X His tag® products (38) ...

Description

Rabbit polyclonal to 6X His tag® - ChIP Grade

Specificity

ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.

Tested applications

IP, WB, IHC-P, ICC/IF, ELISA, ChIP, Flow Cytmore details

Immunogen

Synthetic peptide: HHHHHH conjugated to KLH.

HHHHHH

General notes

This antibody recognizes both C-terminal and N-terminal tags.HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.1% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • ChIP - 6X His tag antibody - ChIP Grade (ab9108)ChIP - 6X His tag antibody - ChIP Grade (ab9108) image (enlarge)

  • Western blot - 6X His tag® antibody - ChIP Grade (ab9108)Western blot - 6X His tag® antibody - ChIP Grade (ab9108) image (enlarge)

  • Western blot - 6X His tag antibody - ChIP Grade (ab9108)Western blot - 6X His tag antibody - ChIP Grade (ab9108) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Cellular localization

Depends upon the localization of the parent protein tagged with hexahistidine.

Alternative names

  • 6 His epitope tag antibody
  • Hexa His tag antibody
  • HHHHHH epitope tag antibody
  • HHHHHH tag antibody
  • His tag antibody
see all

Anti-6X His tag® antibody - ChIP Grade images:

  ChIP - 6X His tag antibody - ChIP Grade (ab9108)

ChIP - 6X His tag antibody - ChIP Grade (ab9108)

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

  Western blot - 6X His tag® antibody - ChIP Grade (ab9108)

Western blot - 6X His tag® antibody - ChIP Grade (ab9108)

Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate

  Western blot - 6X His tag antibody - ChIP Grade (ab9108)

Western blot - 6X His tag antibody - ChIP Grade (ab9108)

All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution

Lane 1 : 10ug non-transformed lysate
Lane 2 : 10ug non-transformed lysate + 1mM IPTG
Lane 3 : 10ug E.coli Lysate harbouring His-tag-RelA NTD, non-induced
Lane 4 : 10ug Induction of His-tag RelA NTD 1 mM IPTG
Lane 5 : 10ug Induction of His-tag RelA NTD 1 mM IPTG

Secondary
HRP conjugated goat anti-rabbit IgG
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 12 kDa (possible non-specific binding).

Exposure time : 1 minute

This image is courtesy of an Abreview.

See Abreview

References for Anti-6X His tag® antibody - ChIP Grade (ab9108)

This product has been referenced in:

  • Milenkovic VMet al. Interaction of Bestrophin-1 and Ca Channel ß-Subunits: Identification of New Binding Domains on the Bestrophin-1 C-Terminus. PLoS One 6:e19364 (2011).Read more (PubMed: 21559412) »
  • Cattepoel Set al. Chronic intranasal treatment with an anti-Aß(30-42) scFv antibody ameliorates amyloid pathology in a transgenic mouse model of Alzheimer's disease. PLoS One 6:e18296 (2011). ICC/IF, IHC-P.Read more (PubMed: 21483675) »

See all 19 publications for this product

Publishing research using ab9108? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"