Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746)

Overview

  • Product name
    Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [HIS-1] to 6X His tag® (Alkaline Phosphatase)
  • Conjugation
    Alkaline Phosphatase
  • Specificity
    ab49746 recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • Tested applications
    Suitable for: WB, Dot blot, ELISAmore details
  • Immunogen

    Recombinant poly(Histidine)-tagged fusion protein.

  • Positive control
    • Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

    The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Primary antibody notes
    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
  • Clonality
    Monoclonal
  • Clone number
    HIS-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 7 kDa.
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746)

This product has been referenced in:
  • Henche AL  et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). Read more (PubMed: 25102813) »
  • Hussack G  et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . Read more (PubMed: 21216961) »

See all 2 Publications for this product

Product Wall

The 6x His-tag should essentially be the same no matter which vector it is expressed by. The affinity of the antibody is more likely to be affected by the proximity of the His-tag to the recombinant protein (N- or C-terminal or within the reading frame...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell)
Loading amount
10 µg
Specification
neuronal cell
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up