Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746)

Overview

  • Product nameAnti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase)See all 6X His tag® primary antibodies ...
  • Description
    Mouse monoclonal [HIS-1] to 6X His tag® (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • Conjugation notesab49746 is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma
  • Specificityab49746 recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • Tested applicationsWB, Dot Blot, ELISAmore details
  • Immunogen

    Recombinant poly(Histidine)-tagged fusion protein.

  • Positive control
    • Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.


    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Purification notesImmunoglobulin fraction purified from ascites fluid.
  • Primary antibody notes Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
  • Clonality Monoclonal
  • Clone numberHIS-1
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab49746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 7 kDa.
Dot Blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746)

This product has been referenced in:
  • Hussack G  et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . Read more (PubMed: 21216961) »

See 1 Publication for this product

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The 6x His-tag should essentially be the same no matter which vector it is expressed by. The affinity of the antibody is more likely to be affected by the proximity of the His-tag to the recombinant protein (N...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal cell)
Loading amount 10 µg
Specification neuronal cell
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"