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Recombinant poly(Histidine)-tagged fusion protein.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma
Our Abpromise guarantee covers the use of ab49746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 7 kDa.|
|Dot blot||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|