Products:Tags & Cell Markers >> Epitope Tags >> His Tag
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ab18184 has been referenced in 14 publications.
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Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.
Left hand panel: untransfected control
Right hand panel: transfected.
Counterstained with DAPI (blue).
All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 293 cells, untransfected control
Lane 2 : 293 cells transfected with His-tagged vector
Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µg
Secondary
An HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilution
This image is courtesy of an anonymous Abreview
ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
This image is courtesy of an anonymous abreview.
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